Forensic DNA Extraction Strategies for PCR Analysis

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Description

There is a transition nationwide on the analysis of forensic evidentiary stains containing biological material from traditional serology to Polymerase Chain Reaction (PCR) methodologies. The increased sensitivity of PCR, the limited number of alleles at each locus, and the necessity of producing unambiguous data for entry into the FBI's Combined DNA Index System make this study of extraction procedures of utmost importance. A "single tube" extraction procedure for blood stains collected onto FTA™ paper and a modified differential nonorganic extraction method from spermatozoa containing mixed stains were analyzed and compared. The extraction success was evaluated by amplification and typing of ... continued below

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xi, 107 leaves : ill.

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Van Winkle, Carolyn May 1998.

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This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided by UNT Libraries to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 42 times . More information about this thesis can be viewed below.

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  • Van Winkle, Carolyn

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Description

There is a transition nationwide on the analysis of forensic evidentiary stains containing biological material from traditional serology to Polymerase Chain Reaction (PCR) methodologies. The increased sensitivity of PCR, the limited number of alleles at each locus, and the necessity of producing unambiguous data for entry into the FBI's Combined DNA Index System make this study of extraction procedures of utmost importance. A "single tube" extraction procedure for blood stains collected onto FTA™ paper and a modified differential nonorganic extraction method from spermatozoa containing
mixed stains were analyzed and compared. The extraction success was evaluated by amplification and typing of the amplified fragment length polymorphism, D1S80. These modifications of the nonorganic method utilized gave an improved separation of the spermatozoa-containing mixed stains.

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xi, 107 leaves : ill.

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UNT Theses and Dissertations

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  • May 1998

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  • March 24, 2014, 8:07 p.m.

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  • Sept. 25, 2014, 11:18 a.m.

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Citations, Rights, Re-Use

Van Winkle, Carolyn. Forensic DNA Extraction Strategies for PCR Analysis, thesis, May 1998; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc278269/: accessed August 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .