Regulation of Escherichia coli pyrBI Gene Expression in Pseudomonas fluorescens

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Pseudomonas fluorescens does not appear to regulate the enzymes of de novo pyrimidine biosynthesis at the level of gene expression. Little or no apparent repression of pyr gene expression is observed upon addition of exogenous pyrimidines to the growth medium. The Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) were sized down and cloned into the broad host range plasmid, pKT230. Upon introduction into a P.fluorescenspyrB mutant strain, ATCase showed repression in response to exogenously fed pyrimidine compounds. Thus, it was possible to bring about changes in pyrimidine nucleotide pool levels and in transcriptional regulation of gene expression at the ... continued below

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vii, 84 leaves : ill.

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Shen, Weiping May 1995.

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  • Shen, Weiping

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Pseudomonas fluorescens does not appear to regulate the enzymes of de novo
pyrimidine biosynthesis at the level of gene expression. Little or no apparent repression of pyr gene expression is observed upon addition of exogenous pyrimidines to the growth medium. The Escherichia coli pyrBI genes for aspartate transcarbamoylase (ATCase) were sized down and cloned into the broad host range plasmid, pKT230. Upon introduction into a P.fluorescenspyrB mutant strain, ATCase showed repression in response to exogenously fed pyrimidine compounds. Thus, it was possible to bring about changes in pyrimidine nucleotide pool levels and in transcriptional regulation of gene expression at the same time.

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vii, 84 leaves : ill.

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UNT Theses and Dissertations

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  • May 1995

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  • March 24, 2014, 8:07 p.m.

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  • June 26, 2014, 9:30 a.m.

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Shen, Weiping. Regulation of Escherichia coli pyrBI Gene Expression in Pseudomonas fluorescens, thesis, May 1995; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc278188/: accessed September 26, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .