Identification and Characterization of the Pyrimidine Biosynthetic Operon in Streptomyces griseus

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To further understand the ATCase/DHOase bifunctional complex formed in Streptomyces, the genes encoding these and other pyrimidine enzymes were identified and characterized. Polymerase chain reaction (PCR) was utilized in this effort. Primers were constructed by selecting conserved regions of pyrimidine genes from known gene and protein sequences of a wide variety of organisms. These sequences were then optimized to Streptomyces codon usage. PCR products were obtained from internal sites within pyrimidine genes and also from primer combinations of different genes. The size, orientation, and partial sequence of the resulting products shows that Streptomyces has a gene organization of pyrR followed ... continued below

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v, 78 leaves : ill.

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Hooten, Jody J. (Jody Jeran) May 1998.

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  • Hooten, Jody J. (Jody Jeran)

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To further understand the ATCase/DHOase bifunctional complex formed in Streptomyces, the genes encoding these and other pyrimidine enzymes were identified and characterized. Polymerase chain reaction (PCR) was utilized in this effort. Primers were constructed by selecting conserved regions of
pyrimidine genes from known gene and protein sequences of a wide variety of organisms. These sequences were then optimized to Streptomyces codon usage. PCR products were obtained from internal sites within pyrimidine genes and also from primer combinations of different genes. The size,
orientation, and partial sequence of the resulting products shows that Streptomyces has a gene organization of pyrR followed by pyrB, pyrC, carA, carB, and pyrF in an operon similar to that found in other Gram-positive bacteria.

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v, 78 leaves : ill.

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  • May 1998

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  • March 24, 2014, 8:07 p.m.

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  • Sept. 24, 2014, 3:43 p.m.

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Hooten, Jody J. (Jody Jeran). Identification and Characterization of the Pyrimidine Biosynthetic Operon in Streptomyces griseus, thesis, May 1998; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc277975/: accessed November 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .