Site Directed Mutagenesis of Dienelactone Hydrolase

PDF Version Also Available for Download.

Description

The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHi to ZscoRI subclone in the plasmid pUC19.

Physical Description

vi, 87 leaves : ill.

Creation Information

Al-Khatib, Haifa Yousef August 1995.

Context

This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided by UNT Libraries to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 76 times , with 4 in the last month . More information about this thesis can be viewed below.

Who

People and organizations associated with either the creation of this thesis or its content.

Chair

Committee Members

Publisher

Rights Holder

For guidance see Citations, Rights, Re-Use.

  • Al-Khatib, Haifa Yousef

Provided By

UNT Libraries

The UNT Libraries serve the university and community by providing access to physical and online collections, fostering information literacy, supporting academic research, and much, much more.

Contact Us

What

Descriptive information to help identify this thesis. Follow the links below to find similar items on the Digital Library.

Degree Information

Description

The clcD gene encoding dienelactone hydrolase (DLH) is part of the clc gene cluster for the utilization of the B-ketoadipate pathway intermediate chlorocatechol. The roles that individual amino acids residues play in catalysis and binding of the enzyme were investigated. Using PCR a 1.9 kbp clcD fragment was amplified and subcloned yielding a 821 bp BamHi to ZscoRI subclone in the plasmid pUC19.

Physical Description

vi, 87 leaves : ill.

Subjects

Language

Identifier

Unique identifying numbers for this thesis in the Digital Library or other systems.

Collections

This thesis is part of the following collection of related materials.

UNT Theses and Dissertations

Theses and dissertations represent a wealth of scholarly and artistic content created by masters and doctoral students in the degree-seeking process. Some ETDs in this collection are restricted to use by the UNT community.

What responsibilities do I have when using this thesis?

When

Dates and time periods associated with this thesis.

Creation Date

  • August 1995

Added to The UNT Digital Library

  • March 24, 2014, 8:07 p.m.

Description Last Updated

  • July 11, 2014, 8:38 a.m.

Usage Statistics

When was this thesis last used?

Yesterday: 1
Past 30 days: 4
Total Uses: 76

Interact With This Thesis

Here are some suggestions for what to do next.

Start Reading

PDF Version Also Available for Download.

International Image Interoperability Framework

IIF Logo

We support the IIIF Presentation API

Al-Khatib, Haifa Yousef. Site Directed Mutagenesis of Dienelactone Hydrolase, thesis, August 1995; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc277940/: accessed October 15, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .