Responses of Cultured Neuronal Networks to the Cannabinoid Mimetic Anandamide

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The effects of cannabinoid agonists on spontaneous neuronal network activity were characterized in murine spinal cord and auditory cortical cultures with multichannel extracellular recording using photoetched electrode arrays. Different cultures responded reproducibly with global decreases of spiking and bursting to anandamide and methanandamide, but each agonist showed unique minor effects on network activity. The two tissues responded in a tissue-specific manner. Spontaneous activity in spinal tissue was terminated by 1 μM anandamide and 6.1 μM methanandamide. Cortical activity ceased at 3.5 μM and 2.8 μM respectively. Irreversible cessation of activity was observed beyond 8 μM for both tissues and test ... continued below

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viii, 73 leaves : ill.

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Morefield, Samantha I. (Samantha Irene) May 1998.

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  • Morefield, Samantha I. (Samantha Irene)

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The effects of cannabinoid agonists on spontaneous neuronal network activity were characterized in murine spinal cord and auditory cortical cultures with multichannel extracellular recording using photoetched electrode arrays. Different cultures responded reproducibly with global decreases of spiking and bursting to anandamide and methanandamide, but each agonist showed unique minor effects on network activity. The two tissues responded in a tissue-specific manner. Spontaneous activity in spinal tissue was terminated by 1 μM anandamide and 6.1 μM methanandamide. Cortical activity ceased at 3.5 μM and 2.8 μM respectively. Irreversible cessation of activity was observed beyond 8 μM for both tissues and test substances. Palmitoylethanolamide, demonstrated that CB2 receptors were not present or not responsive. However, the data strongly suggested the presence of CB1 receptors.

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viii, 73 leaves : ill.

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  • May 1998

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  • March 24, 2014, 8:07 p.m.

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  • Sept. 24, 2014, 11:56 a.m.

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Citations, Rights, Re-Use

Morefield, Samantha I. (Samantha Irene). Responses of Cultured Neuronal Networks to the Cannabinoid Mimetic Anandamide, thesis, May 1998; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc277717/: accessed August 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .