This system will be undergoing maintenance February 20th between 9:00AM and 12:00PM CST.

Role of ribosomal RNA released from red cells in blood coagulation in zebrafish and humans

PDF Version Also Available for Download.

Description

This article identifies that an rRNA released in hemolysis activates clotting in human and zebrafish plasma. Furthermore, it shows that fish Hgfac plays a role in rRNA-mediated activation of coagulation.

Physical Description

14 p.

Creation Information

Alharbi, Abdulmajeed; Iyer, Neha; Al Qaryoute, Ayah; Raman, Revathi; Burks, David J.; Azad, Rajeev K. et al. November 17, 2021.

Context

This article is part of the collection entitled: UNT Scholarly Works and was provided by the UNT College of Science to the UNT Digital Library, a digital repository hosted by the UNT Libraries. More information about this article can be viewed below.

Who

People and organizations associated with either the creation of this article or its content.

Authors

Provided By

UNT College of Science

The College of Science provides students with the high-demand skills and knowledge to succeed as researchers and professionals. The College includes four departments: Biology, Chemistry, Math, and Physics, and is also home to a number of interdisciplinary programs, centers, institutes, intercollegiate programs, labs, and services.

Contact Us

What

Descriptive information to help identify this article. Follow the links below to find similar items on the Digital Library.

Degree Information

Description

This article identifies that an rRNA released in hemolysis activates clotting in human and zebrafish plasma. Furthermore, it shows that fish Hgfac plays a role in rRNA-mediated activation of coagulation.

Physical Description

14 p.

Notes

Abstract: Hemolytic disorders are characterized by hemolysis and are prone to thrombosis. It has previously been shown that the RNA released from damaged blood cells activates clotting. However, the nature of the RNA released from hemolysis is still elusive. We found that after hemolysis, red blood cells from both zebrafish and humans released RNA that contained mostly 5.8S ribosomal RNA (5.8S rRNA), This RNA activated coagulation in zebrafish and human plasmas. By using both natural and synthetic 5.8S rRNA and its truncated fragments, we found that the 3'-end 26-nucleotide-long RNA (3'-26 RNA) and its stem-loop secondary structure were necessary and sufficient for clotting activity. Corn trypsin inhibitor (CTI), a coagulation factor XII (FXII) inhibitor, blocked 3'-26 RNA–mediated coagulation activation in the plasma of both zebrafish and humans. CTI also inhibited zebrafish coagulation in vivo. 5.8S rRNA monoclonal antibody inhibited both 5.8S rRNA– and 3'-26 RNA–mediated zebrafish coagulation activity. Both 5.8S rRNA and 3'-26 RNA activated normal human plasma but did not activate FXII-deficient human plasma. Taken together, these results suggested that the activation of zebrafish plasma is via an FXII-like protein. Because zebrafish have no FXII and because hepatocyte growth factor activator (Hgfac) has sequence similarities to FXII, we knocked down the hgfac in adult zebrafish. We found that plasma from this knockdown fish does not respond to 3'-26 RNA. To summarize, we identified that an rRNA released in hemolysis activates clotting in human and zebrafish plasma. Furthermore, we showed that fish Hgfac plays a role in rRNA-mediated activation of coagulation.

Key Points: Hemolysis releases 5.8S rRNA and activates blood coagulation in human and zebrafish via FXII and Hgfac, respectively.
Only the 3'-end 26 nucleotides of 5.8S rRNA were necessary and sufficient for this activation.

Source

  • Blood Advances, 5(22), American Society of Hematology, November 17, 2021, pp. 1-14

Language

Item Type

Identifier

Unique identifying numbers for this article in the Digital Library or other systems.

Publication Information

  • Publication Title: Blood Advances
  • Volume: 5
  • Issue: 22
  • Page Start: 4634
  • Page End: 4647
  • Peer Reviewed: Yes

Collections

This article is part of the following collection of related materials.

UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

What responsibilities do I have when using this article?

When

Dates and time periods associated with this article.

Creation Date

  • November 17, 2021

Added to The UNT Digital Library

  • Jan. 14, 2022, 4:33 p.m.

Description Last Updated

  • Nov. 20, 2023, 1:38 p.m.

Usage Statistics

When was this article last used?

Yesterday: 0
Past 30 days: 0
Total Uses: 5

Interact With This Article

Here are some suggestions for what to do next.

Start Reading

PDF Version Also Available for Download.

International Image Interoperability Framework

IIF Logo

We support the IIIF Presentation API

Alharbi, Abdulmajeed; Iyer, Neha; Al Qaryoute, Ayah; Raman, Revathi; Burks, David J.; Azad, Rajeev K. et al. Role of ribosomal RNA released from red cells in blood coagulation in zebrafish and humans, article, November 17, 2021; (https://digital.library.unt.edu/ark:/67531/metadc1877585/: accessed February 19, 2025), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT College of Science.

Back to Top of Screen