Comparative Immunity Analysis in Mycobacterium smegmatis and Mycobacteriophage

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Paper describes research conducted with the goal of expanding the body of information related to mycobacteriophages and the relationship between those viruses and the bacteria that are immune to them.

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13 p.: ill.

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Schade, Amy E. 2012.

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This article is part of the collection entitled: The Eagle Feather and was provided by the UNT Libraries to the UNT Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 45 times. More information about this article can be viewed below.

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Paper describes research conducted with the goal of expanding the body of information related to mycobacteriophages and the relationship between those viruses and the bacteria that are immune to them.

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13 p.: ill.

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Abstract: The purpose of this research is to expand the body of information related to mycobacteriophages and the relationship between those viruses and the bacteria that are immune to them. In this project the researchers used Mycobacterium smegmatis as the host bacteria as it is from the same genus as the organisms that cause tuberculosis and leprosy but is innocuous to humans. This research used mycobacteriophages isolated from the National Genomics Research Initiative classes at UNT from 2009 and 2010 which are members of a variety of clusters and subclusters. The lysogenic pathway was the focus of the project because it involves the insertion of the mycobacteriophage’s DNA onto the bacterial chromosome. To isolate bacteria that are immune to certain mycobacteriophages, the bacteria were infected by the phage, and mesas were allowed to form. A mesa is a film of growth that occurs on top of a zone of clearing established by mycobacteriophages. A small amount was picked from the mesa and then streaked for isolation. After growing this colony into a large culture batch, immunity assays were performed which involved infecting the lysogen containing the phage against the 53 phages isolated from UNT. Some interesting immunity patterns were observed. The lysogen of the mycobacteriophage Adephagia, named Adephagia L1, is immune to the phage Lew as well as nine other phages. Lew L1 is immune to Adephagia and those same nine phages as well as the phage, Tootziepop. While their lysogens are immune to each other’s phage, they do not share the immunity to Tootziepop. This means there was some change at the DNA level during the start of the lysogenic life pathway. Future research will focus on why immunity is or is not transferred. Many of the lysogens isolated were immune to only one phage which means they are likely very unique. Information from this project can help aid in the decision on which phage should be sequenced next, as well as provide a means to examine how the phage genome is transferred into the bacteria.

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  • Eagle Feather, Issue 9, University of North Texas Honors College: Denton, Texas. 2012

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  • Publication Title: Eagle Feather
  • Volume: 9
  • Issue: 2012
  • Peer Reviewed: Yes

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The Eagle Feather

Launched in 2004 by UNT's Honors College, The Eagle Feather was an interdisciplinary undergraduate research journal that promoted the work of students and their faculty mentors. The Eagle Feather was published annually until 2017 when it transitioned into the North Texas Journal of Undergraduate Research.

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This collection presents scholarly and artistic content created by undergraduate students. All materials have been previously accepted by a professional organization or approved by a faculty mentor. Most classroom assignments are not eligible for inclusion. The collection includes, but is not limited to Honors College theses, thesis supplemental files, professional presentations, articles, and posters. Some items in this collection are restricted to use by the UNT community.

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  • 2012

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  • April 22, 2020, 5:45 p.m.

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  • May 11, 2020, 1:54 p.m.

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Schade, Amy E. Comparative Immunity Analysis in Mycobacterium smegmatis and Mycobacteriophage, article, 2012; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc1635164/: accessed May 24, 2025), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; .

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