01-ERD-111 - The Development of Synthetic High Affinity Ligands

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The aim of this project was to develop Synthetic High-Affinity Ligands (SHALs), which bind with high affinity and specificity to proteins of interest for national security and cancer therapy applications. The aim of producing synthetic ligands for sensory devices as an alternative to antibody-based detection assays and therapeutic agents is to overcome the drawbacks associated with antibody-based in next-generation sensors and systems. The focus area of the project was the chemical synthesis of the SHALs. The project concentrated on two different protein targets. (a) The C fragment of tetanus and botulinum toxin, potential biowarfare agents. A SHAL for tetanus or ... continued below

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PDF-file: 5 pages; size: 98 Kbytes

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Perkins, J; Balhorn, R; Cosman, M; Lightstone, F & Zeller, L February 5, 2004.

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Description

The aim of this project was to develop Synthetic High-Affinity Ligands (SHALs), which bind with high affinity and specificity to proteins of interest for national security and cancer therapy applications. The aim of producing synthetic ligands for sensory devices as an alternative to antibody-based detection assays and therapeutic agents is to overcome the drawbacks associated with antibody-based in next-generation sensors and systems. The focus area of the project was the chemical synthesis of the SHALs. The project concentrated on two different protein targets. (a) The C fragment of tetanus and botulinum toxin, potential biowarfare agents. A SHAL for tetanus or botulinum toxin would be incorporated into a sensory device for the toxins. (b) HLA-DR10, a protein found in high abundance on the surface of Non-Hodgkins Lymphoma. A SHAL specific to a tumor marker, labeled with a radionuclide, would enable the targeted delivery of radiation therapy to metastatic disease. The technical approach used to develop a SHAL for each protein target will be described in more detail below. However, in general, the development of a SHAL requires a combination of computational modeling techniques, modern nuclear magnetic resonance spectroscopy (NMR) and synthetic chemistry.

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PDF-file: 5 pages; size: 98 Kbytes

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  • Report No.: UCRL-TR-202374
  • Grant Number: W-7405-ENG-48
  • DOI: 10.2172/15013987 | External Link
  • Office of Scientific & Technical Information Report Number: 15013987
  • Archival Resource Key: ark:/67531/metadc1404716

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • February 5, 2004

Added to The UNT Digital Library

  • Jan. 23, 2019, 12:54 p.m.

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  • Jan. 25, 2019, 12:47 p.m.

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Perkins, J; Balhorn, R; Cosman, M; Lightstone, F & Zeller, L. 01-ERD-111 - The Development of Synthetic High Affinity Ligands, report, February 5, 2004; Livermore, California. (https://digital.library.unt.edu/ark:/67531/metadc1404716/: accessed August 22, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.