Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil

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This article proposes a model for the accumulation of substantial levels of cyclopropane fatty acids (CPA) in camelina, and discusses the differences between CPA accumulation in camelina and Arabidopsis.

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13 p.

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Yu, Xiao-Hong; Cahoon, Rebecca E.; Horn, Patrick J.; Shi, Hai; Prakash, Richa R.; Cai, Yuanheng et al. September 20, 2017.

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This article proposes a model for the accumulation of substantial levels of cyclopropane fatty acids (CPA) in camelina, and discusses the differences between CPA accumulation in camelina and Arabidopsis.

Physical Description

13 p.

Notes

Abstract: Modified fatty acids (mFA) have diverse uses; for example, cyclopropane fatty acids (CPA) are
feedstocks for producing coatings, lubricants, plastics and cosmetics. The expression of mFAproducing
enzymes in crop and model plants generally results in lower levels of mFA
accumulation than in their natural-occurring source plants. Thus, to further our understanding of
metabolic bottlenecks that limit mFA accumulation, we generated transgenic Camelina sativa
lines co-expressing Escherichia coli cyclopropane synthase (EcCPS) and Sterculia foetida
lysophosphatidic acid acyltransferase (SfLPAT). In contrast to transgenic CPA-accumulating
Arabidopsis, CPA accumulation in camelina caused only minor changes in seed weight,
germination rate, oil accumulation and seedling development. CPA accumulated to much higher
levels in membrane than storage lipids, comprising more than 60% of total fatty acid in both
phosphatidylcholine (PC) and phosphatidylethanolamine (PE) versus 26% in diacylglycerol (DAG)
and 12% in triacylglycerol (TAG) indicating bottlenecks in the transfer of CPA from PC to DAG
and from DAG to TAG. Upon co-expression of SfLPAT with EcCPS, di-CPA-PC increased by
~50% relative to lines expressing EcCPS alone with the di-CPA-PC primarily observed in the
embryonic axis and mono-CPA-PC primarily in cotyledon tissue. EcCPS-SfLPAT lines revealed a
redistribution of CPA from the sn-1 to sn-2 positions within PC and PE that was associated with a
doubling of CPA accumulation in both DAG and TAG. The identification of metabolic
bottlenecks in acyl transfer between site of synthesis (phospholipids) and deposition in storage
oils (TAGs) lays the foundation for the optimizing CPA accumulation through directed
engineering of oil synthesis in target crops.

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  • Plant Biotechnology Journal, 2018. Hoboken, NJ: John Wiley & Sons

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  • Publication Title: Plant Biotechnology Journal
  • Volume: 16
  • Page Start: 926
  • Page End: 938
  • Peer Reviewed: Yes

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  • July 10, 2017

Accepted Date

  • September 14, 2017

Creation Date

  • September 20, 2017

Added to The UNT Digital Library

  • Jan. 12, 2019, 5:20 p.m.

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Yu, Xiao-Hong; Cahoon, Rebecca E.; Horn, Patrick J.; Shi, Hai; Prakash, Richa R.; Cai, Yuanheng et al. Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil, article, September 20, 2017; Warwickshire, United Kingdom. (https://digital.library.unt.edu/ark:/67531/metadc1404208/: accessed June 19, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; crediting UNT College of Arts and Sciences.