Recent developments with the human repair genes ERCC2, ERCC4, and XRCC1

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ERCC2 was first identified as a gene on human chromosome 19 that complemented the UV sensitivity of CHO UV5 cells in somatic cell hybrids. Subsequent studies localized ERCC2 to the same chromosomal region (19q13.2--13.3) as the ERCC1 gene and showed that the two genes were less than 250 kb apart. Cloning of ERCC2 was accomplished by transfection of genomic DNA into UV5 cells and rescue of the gene from a secondary transformant. Recovery of the gene was aided by the presence of repetitive sequences that were detected on Southern blots with a probe for Alu-family repeats. ERCC2, which is 19 ... continued below

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17 p.

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Thompson, L. H.; Caldecott, K. W.; Brookman, K. W.; Weber, C. A.; Salazar, E. S.; Takayama, K. et al. November 6, 1992.

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ERCC2 was first identified as a gene on human chromosome 19 that complemented the UV sensitivity of CHO UV5 cells in somatic cell hybrids. Subsequent studies localized ERCC2 to the same chromosomal region (19q13.2--13.3) as the ERCC1 gene and showed that the two genes were less than 250 kb apart. Cloning of ERCC2 was accomplished by transfection of genomic DNA into UV5 cells and rescue of the gene from a secondary transformant. Recovery of the gene was aided by the presence of repetitive sequences that were detected on Southern blots with a probe for Alu-family repeats. ERCC2, which is 19 kb in size, quantitatively corrected the UV sensitivity and incision defect in UV5 cells upon transfection. An ERCC2 CDNA clone was recovered from the pcD2 expression library. Although this clone was truncated at the 5 in. end, it conferred transient, but not stable, correction to UV5 cells upon transfection. Based on genomic sequence, this clone was extended by oligonucleotide addition to obtain minigene constructs in which the complete open reading frame (ORF) was present. Translation of the ERCC2 ORF gives an amino acid sequence that has 72% similarity with the S. cerevisiae RAD3 protein, which encodes a DNA helicase.

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17 p.

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OSTI; NTIS; GPO Dep.

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  • Alfred Benzon symposium 35 on DNA repair mechanism,Copenhagen (Denmark),8-13 Aug 1992

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  • Other: DE93017159
  • Report No.: UCRL-JC--113127
  • Report No.: CONF-9208143--2
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 10173359
  • Archival Resource Key: ark:/67531/metadc1389364

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  • November 6, 1992

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  • Nov. 28, 2018, 2:33 p.m.

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  • Dec. 6, 2018, 3:26 p.m.

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Thompson, L. H.; Caldecott, K. W.; Brookman, K. W.; Weber, C. A.; Salazar, E. S.; Takayama, K. et al. Recent developments with the human repair genes ERCC2, ERCC4, and XRCC1, article, November 6, 1992; California. (digital.library.unt.edu/ark:/67531/metadc1389364/: accessed January 20, 2019), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.