[Nonhomologous mechanisms of repair of chromosomal breaks]. Progress report

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Broken chromosomes must either be repaired or lost. The break separates part of the chromosome, containing a telomere, from the rest, containing a centromere. While the centromerecontaining fragment can properly segregate, the broken end will be progressively degraded. The acentric fragment cannot segregate and will also be degraded. We have centered our attention on two alternative non-homologous mechanisms of repair: (1) the acquisition of a new telomere, and (2) repair of broken chromosomes by non-homologous joining of broken chromosome ends. In both cases, we create a double-strand break at a defined chromosomal location in yeast cells. The break is created ... continued below

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14 p.

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Haber, J. E. September 1, 1993.

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Description

Broken chromosomes must either be repaired or lost. The break separates part of the chromosome, containing a telomere, from the rest, containing a centromere. While the centromerecontaining fragment can properly segregate, the broken end will be progressively degraded. The acentric fragment cannot segregate and will also be degraded. We have centered our attention on two alternative non-homologous mechanisms of repair: (1) the acquisition of a new telomere, and (2) repair of broken chromosomes by non-homologous joining of broken chromosome ends. In both cases, we create a double-strand break at a defined chromosomal location in yeast cells. The break is created by the site-specific HO endonuclease in cells that carry the rad52 mutation to prevent repair of a double-strand break by homologous recombination. In diploid cells, we can recover cells that contain a terminally deleted, healed chromosome that has acquired a new telomere. In haploid cells, we can recover cells in which the double-strand break has been repaired by rejoining the broken ends, usually accompanied by a deletion.

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14 p.

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OSTI; NTIS; GPO Dep.

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  • Other Information: PBD: [1993]

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  • Other: DE93019986
  • Report No.: DOE/ER/61235--T1
  • Grant Number: FG02-91ER61235
  • DOI: 10.2172/10176518 | External Link
  • Office of Scientific & Technical Information Report Number: 10176518
  • Archival Resource Key: ark:/67531/metadc1386268

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  • September 1, 1993

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  • Nov. 28, 2018, 2:33 p.m.

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Haber, J. E. [Nonhomologous mechanisms of repair of chromosomal breaks]. Progress report, report, September 1, 1993; United States. (https://digital.library.unt.edu/ark:/67531/metadc1386268/: accessed March 26, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.