The use of dimorphic Alu insertions in human DNA fingerprinting

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We have characterized certain Human Specific Alu Insertions as either dimorphic (TPA25, PV92, APO), sightly dimorphic (C2N4 and C4N4) or monomorphic (C3N1, C4N6, C4N2, C4N5, C4N8), based on studies of Caucasian, Asian, American Black and African Black populations. Our approach is based upon: (1) PCR amplification using primers directed to the sequences that flank the site of insertion of the different Alu elements studied; (2) gel electrophoresis and scoring according to the presence or absence of an Alu insertion in one or both homologous chromosomes; (3) allelic frequencies calculated and compared according to Hardy-Weinberg equilibrium. Our DNA fingerprinting procedure using ... continued below

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17 p.

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Novick, G. E.; Gonzalez, T.; Garrison, J.; Novick, C. C.; Herrera, R. J.; Batzer, M. A. et al. December 4, 1992.

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We have characterized certain Human Specific Alu Insertions as either dimorphic (TPA25, PV92, APO), sightly dimorphic (C2N4 and C4N4) or monomorphic (C3N1, C4N6, C4N2, C4N5, C4N8), based on studies of Caucasian, Asian, American Black and African Black populations. Our approach is based upon: (1) PCR amplification using primers directed to the sequences that flank the site of insertion of the different Alu elements studied; (2) gel electrophoresis and scoring according to the presence or absence of an Alu insertion in one or both homologous chromosomes; (3) allelic frequencies calculated and compared according to Hardy-Weinberg equilibrium. Our DNA fingerprinting procedure using PCR amplification of dimorphic Human Specific Alu insertions, is stable enough to be used not only as a tool for genetic mapping but also to characterize populations, study migrational patterns and track the inheritance of human genetic disorders.

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17 p.

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OSTI; NTIS; GPO Dep.

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  • 2. international symposium on DNA fingerprinting,Belo Horizonte (Brazil),9 Nov 1992

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  • Other: DE93016530
  • Report No.: UCRL-JC--112318
  • Report No.: CONF-9211249--1
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 10170149
  • Archival Resource Key: ark:/67531/metadc1341085

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  • December 4, 1992

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  • Nov. 28, 2018, 2:33 p.m.

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  • Dec. 6, 2018, 2:04 p.m.

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Novick, G. E.; Gonzalez, T.; Garrison, J.; Novick, C. C.; Herrera, R. J.; Batzer, M. A. et al. The use of dimorphic Alu insertions in human DNA fingerprinting, article, December 4, 1992; California. (digital.library.unt.edu/ark:/67531/metadc1341085/: accessed January 22, 2019), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.