A method of genetically manipulating an acidophilic bacteria is provided by two different procedures. Using electroporation, chimeric and broad-host range plasmids are introduced into Acidiphilium. Conjugation is also employed to introduce broad-host range plasmids into Acidiphilium at neutral pH.
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EG and G Idaho, Inc., Idaho Falls, ID (United States)
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Idaho Falls, Idaho
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A method of genetically manipulating an acidophilic bacteria is provided by two different procedures. Using electroporation, chimeric and broad-host range plasmids are introduced into Acidiphilium. Conjugation is also employed to introduce broad-host range plasmids into Acidiphilium at neutral pH.
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Roberto, F. F.; Glenn, A. W. & Ward, T. E.A method of genetically engineering acidophilic, heterotrophic, bacteria by electroporation and conjugation,
patent,
August 7, 1990;
Idaho Falls, Idaho.
(https://digital.library.unt.edu/ark:/67531/metadc1315704/:
accessed April 23, 2024),
University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu;
crediting UNT Libraries Government Documents Department.