DNA sequencing by a single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA

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Description

We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence.

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6 p.

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Goodwin, P. M.; Schecker, J. A.; Wilkerson, C. W.; Hammond, M. L.; Ambrose, W. P.; Jett, J. H. et al. February 1, 1993.

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Description

We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence.

Physical Description

6 p.

Notes

OSTI; NTIS; GPO Dep.

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  • OE/LASE `93: International Society for Optical Engineering (SPIE) conference,Los Angeles, CA (United States),13-23 Jan 1993

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  • Other: DE93007331
  • Report No.: LA-UR--93-362
  • Report No.: CONF-930159--8
  • Grant Number: W-7405-ENG-36
  • Office of Scientific & Technical Information Report Number: 10131004
  • Archival Resource Key: ark:/67531/metadc1313592

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  • February 1, 1993

Added to The UNT Digital Library

  • Nov. 3, 2018, 11:47 a.m.

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  • Nov. 12, 2018, 8:42 p.m.

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Goodwin, P. M.; Schecker, J. A.; Wilkerson, C. W.; Hammond, M. L.; Ambrose, W. P.; Jett, J. H. et al. DNA sequencing by a single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA, article, February 1, 1993; New Mexico. (https://digital.library.unt.edu/ark:/67531/metadc1313592/: accessed April 26, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.