Evaluation of Mitotic Time in Vivo, Using Tritiated Thymidine as a Cell Marker: Successive Labeling with Time of Separate MItotic Phases

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The duration of mitosis is of great importance in an evaluation of growth rates in proliferating somatic tissues, since calculations are based on observations of mitotic activity, and therefore final results are directly proportional to an assumed value for mitotic time. This duration cannot be measured directly in vivo at a tissue level, since the mode of distribution of the single cell value is not known. This difficulty is not overcome in extrapolations from in vitro measurements. Also it is not overcome with the labeling of proliferating cells after tritiated thymidine injection in vivo if subsequent observation is limited to … continued below

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Odartchenko, N.; Cottier, H.; Feinendegen, L. E. & Bond, V. P. October 15, 1963.

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  • Main Title: Evaluation of Mitotic Time in Vivo, Using Tritiated Thymidine as a Cell Marker: Successive Labeling with Time of Separate MItotic Phases
  • Series Title: BNL (Series)
  • Series Title: Brookhaven National Laboratory Reports
  • Added Title: Brookhaven National Laboratory Report BNL-7260

Description

The duration of mitosis is of great importance in an evaluation of growth rates in proliferating somatic tissues, since calculations are based on observations of mitotic activity, and therefore final results are directly proportional to an assumed value for mitotic time. This duration cannot be measured directly in vivo at a tissue level, since the mode of distribution of the single cell value is not known. This difficulty is not overcome in extrapolations from in vitro measurements. Also it is not overcome with the labeling of proliferating cells after tritiated thymidine injection in vivo if subsequent observation is limited to the rate of progression into mitosis of labeled cells that incorporated the tracer during the period of DNA-synthesis. Observation of separate mitotic phases, however, offers the possibility of following the progression of the wave of labeled cells at successive, short-lasting checking steps, and to analyze the variability in the times of passage through mitosis. In the present work, the progression of labeled cells as a function of time, after a single injection of tritiated thymidine, was followed in successive phases of mitosis in erythroblasts of dog bone marrow.

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  • October 15, 1963

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  • Feb. 5, 2022, 9:57 a.m.

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Thumbnail image of item number 1 in: 'Evaluation of Mitotic Time in Vivo, Using Tritiated Thymidine as a Cell Marker: Successive Labeling with Time of Separate MItotic Phases'.
Thumbnail image of item number 2 in: 'Evaluation of Mitotic Time in Vivo, Using Tritiated Thymidine as a Cell Marker: Successive Labeling with Time of Separate MItotic Phases'.
Thumbnail image of item number 3 in: 'Evaluation of Mitotic Time in Vivo, Using Tritiated Thymidine as a Cell Marker: Successive Labeling with Time of Separate MItotic Phases'.
Thumbnail image of item number 4 in: 'Evaluation of Mitotic Time in Vivo, Using Tritiated Thymidine as a Cell Marker: Successive Labeling with Time of Separate MItotic Phases'.

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Odartchenko, N.; Cottier, H.; Feinendegen, L. E. & Bond, V. P. Evaluation of Mitotic Time in Vivo, Using Tritiated Thymidine as a Cell Marker: Successive Labeling with Time of Separate MItotic Phases, report, October 15, 1963; Washington D.C.. (https://digital.library.unt.edu/ark:/67531/metadc1255868/: accessed December 4, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.

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