A Solution of the Distributional Error Problem in Cytophotometry

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Description

Use of that portion of the characteristic curve of photographic film in which transmission is linear with log exposure eliminates the distributional error and thus makes possible the assessment of total mass of an inhomogeneously distributed cell consistent without scanning or measuring the cell's projected area. Measurement consists of obtaining the difference in output of a photocell receiving the total light transmitted by a photomicrograph of the cell, and a photomicrograph of the microscope field illumination against which the cell was photographed. A method of making such measurements is given, and its basis is described.

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10 pages

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Adams, Lawrence R. & Sondhaus, Charles A. December 19, 1961.

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Description

Use of that portion of the characteristic curve of photographic film in which transmission is linear with log exposure eliminates the distributional error and thus makes possible the assessment of total mass of an inhomogeneously distributed cell consistent without scanning or measuring the cell's projected area. Measurement consists of obtaining the difference in output of a photocell receiving the total light transmitted by a photomicrograph of the cell, and a photomicrograph of the microscope field illumination against which the cell was photographed. A method of making such measurements is given, and its basis is described.

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10 pages

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Digitized from microopaque cards (1).

Includes bibliographic references (page 10)

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  • SuDoc Number: Y 3.At 7:22/UCRL-9994
  • Report No.: UCRL-9994
  • Accession or Local Control No: metadc1255496
  • Archival Resource Key: ark:/67531/metadc1255496

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TRAIL Microcard Collection

Imaged from microcard, these technical reports describe research performed for U.S. government agencies from the 1930s to the 1960s. The reports were provided by the Technical Report Archive and Image Library (TRAIL).

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  • December 19, 1961

Added to The UNT Digital Library

  • Feb. 5, 2022, 9:50 a.m.

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  • Feb. 8, 2023, 5:03 p.m.

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Adams, Lawrence R. & Sondhaus, Charles A. A Solution of the Distributional Error Problem in Cytophotometry, report, December 19, 1961; Livermore, California. (https://digital.library.unt.edu/ark:/67531/metadc1255496/: accessed May 12, 2025), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.

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