Genetic analysis of the spindle checkpoint genes san-I, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans

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Article on a genetic analysis of the spindle checkpoint genes san-I, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans.

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18 p.

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Hajeri, Vinita A.; Stewart, Anil M.; Moore, Landon L. & Padilla, Pamela A. February 4, 2008.

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Article on a genetic analysis of the spindle checkpoint genes san-I, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans.

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18 p.

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Abstract: Background: The spindle checkpoint delays the onset of anaphase until all sister chromatids are aligned properly at the metaphase plate. To investigate the role san-1, the MAD3 homologue, has in Caenorhabditis elegans embryos, the authors used RNA interference (RNAi) to identify genes synthetic lethal with the viable san-1 (ok1580) deletion mutant. Results: The san-1 (ok1580) animal has low penetrating phenotypes including an increased incidence of males, larvae arrest, slow growth, protruding vulva, and defects in vulva morphogenesis. The authors found that the viability of san-1 (ok1580) embryos is significantly reduced when HCP-1 (CENP-F homologue) are reduced by RNAi. Interestingly, the viability of san-1 (ok1580) embryos is not significantly reduced when the paralog of HCP-1, HCP-2, is reduced. The phenotype of san-1 (ok1580); hcp-1 (RNAi) embryos includes embryonic and larval lethality, abnormal organ development, and an increase in abnormal chromosome segregation (abberrant mitotic nuclei, anaphase bridging). Several of the san-1 (ok1580); hcp-1(RNAi) animals displayed abnormal kinetochore (detected by MPM-2) and microtubule structure. The survival of mdf-2 (RNAi); hcp-1 (RNAi) embryos but not bub-3 (RNAi); hcp-1 (RNAi) embryos was also compromised. Finally, the authors found that san-1 (ok1580) and bub-3 (RNAi), but not hcp-1 (RNAi) embryos, were sensitive to anoxia, suggesting that like SAN-1, BUB-3 has a functional role as a spindle checkpoint protein. Conclusion: Together, these data suggest that in the C. elegans embryo, HCP-1 interacts with a subset of the spindle checkpoint pathway. Furthermore, the fact that san-1 (ok1580); hcp-1 (RNAi) animals had a severe viability defect whereas in the san-1 (ok1580); hcp-2 (RNAi) and san-1 (ok1580); hcp-2 (ok1757) animals the viability defect was not as severe suggesting that hcp-1 and hcp-2 are not completely redundant.

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  • Cell Division, 2008, London: BioMed Central Ltd.

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  • Publication Title: Cell Division
  • Volume: 3
  • Issue: 6
  • Peer Reviewed: Yes

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UNT Scholarly Works

The Scholarly Works Collection is home to materials from the University of North Texas community's research, creative, and scholarly activities and serves as UNT's Open Access Repository. It brings together articles, papers, artwork, music, research data, reports, presentations, and other scholarly and creative products representing the expertise in our university community. Access to some items in this collection may be restricted.

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  • February 4, 2008

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  • Nov. 30, 2012, 9:15 a.m.

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  • May 14, 2014, 12:29 p.m.

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Hajeri, Vinita A.; Stewart, Anil M.; Moore, Landon L. & Padilla, Pamela A. Genetic analysis of the spindle checkpoint genes san-I, mdf-2, bub-3 and the CENP-F homologues hcp-1 and hcp-2 in Caenorhabditis elegans, article, February 4, 2008; [London, United Kingdom]. (digital.library.unt.edu/ark:/67531/metadc122165/: accessed May 30, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.