Investigations of mechanisms of microbial enhanced oil recovery by microbes and their metabolic products

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Experiments at NIPER have demonstrated that oil mobilization by microbial formulations is not merely the result of the effects of the metabolic products from the in situ fermentation of nutrient. A combination of two microorganisms, Bacillus licheniformis, NIPER and a Clostridium species (NIPER 6) was determined to be an effective microbial formulation for the recovery of residual crude oil in porous media. Flask tests with various nutrients and environmental conditions were used to evaluate the growth and metabolite production of NIPER 1 and 6. Several interfacial tension (IFT) measurements were conducted using certain metabolic products from the combined microbial cultures ... continued below

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Pages: (27 p)

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Chase, K.L.; Bryant, R.S.; Bertus, K.M. & Stepp, A.K. December 1, 1990.

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Description

Experiments at NIPER have demonstrated that oil mobilization by microbial formulations is not merely the result of the effects of the metabolic products from the in situ fermentation of nutrient. A combination of two microorganisms, Bacillus licheniformis, NIPER and a Clostridium species (NIPER 6) was determined to be an effective microbial formulation for the recovery of residual crude oil in porous media. Flask tests with various nutrients and environmental conditions were used to evaluate the growth and metabolite production of NIPER 1 and 6. Several interfacial tension (IFT) measurements were conducted using certain metabolic products from the combined microbial cultures NIPER 1 and NIPER 6. Nonane was used as the oil for these experiments, since crude oil from Delaware-Childers field emulsifies very easily. The IFT of a selected microbial formulation were measured with two different crude oils using brines of varying salinities. Comparisons were made with saline brines containing only the nutrient and with microbial metabolite solutions from which the active cells have been removed by filtration to isolate the specific effects of the microbial cells. Etched-glass micromodel studies showed that the microbial formulation effectively mobilized crude oil trapped after waterflooding. Wettability alteration and unsteady-state relative permeability tests were performed in Berea Sandstone cores. 16 refs., 17 figs., 12 tabs.

Physical Description

Pages: (27 p)

Notes

NTIS, PC A03/MF A01 - OSTI; GPO Dep.

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  • Other: DE91002210
  • Report No.: NIPER-483
  • Grant Number: FC22-83FE60149
  • DOI: 10.2172/6469838 | External Link
  • Office of Scientific & Technical Information Report Number: 6469838
  • Archival Resource Key: ark:/67531/metadc1212171

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • December 1, 1990

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  • July 5, 2018, 11:11 p.m.

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Chase, K.L.; Bryant, R.S.; Bertus, K.M. & Stepp, A.K. Investigations of mechanisms of microbial enhanced oil recovery by microbes and their metabolic products, report, December 1, 1990; United States. (https://digital.library.unt.edu/ark:/67531/metadc1212171/: accessed March 26, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.