Reconstitution of halorhodopsin

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Halobacterium halobium contains a family of retinal-bound proteins: bacteriorhodopsin (bR) which mediates phototrophic growth as a light-riven proton pump, halorhodopsin (hR) which is a light-driven chloride pump, and one or more sensory rhodopsins (sR) which mediate a phototactic response. Two-dimensional crystallization of halorhodopsin has been attempted though the reconstitution of purified halorhodopsin with purple membrane lipid for electron microscopy work. The first important step for crystallization is to get a homogeneous protein which is pure and not denatured. Homogeneous halorhodopsin has been obtained by a modification of existing purification methods. Some nice looking membrane patches which have the same density ... continued below

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Pages: (83 p)

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Kong, T. November 1, 1989.

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Description

Halobacterium halobium contains a family of retinal-bound proteins: bacteriorhodopsin (bR) which mediates phototrophic growth as a light-riven proton pump, halorhodopsin (hR) which is a light-driven chloride pump, and one or more sensory rhodopsins (sR) which mediate a phototactic response. Two-dimensional crystallization of halorhodopsin has been attempted though the reconstitution of purified halorhodopsin with purple membrane lipid for electron microscopy work. The first important step for crystallization is to get a homogeneous protein which is pure and not denatured. Homogeneous halorhodopsin has been obtained by a modification of existing purification methods. Some nice looking membrane patches which have the same density as purple membrane have been obtained. But unfortunately, they are not crystalline. The procedure of hR reconstitution is described in detail and some other strategies to induce the protein crystal in the reconstituted membrane are discussed in this dissertation. 76 refs., 20 figs., 6 tabs.

Physical Description

Pages: (83 p)

Notes

NTIS, PC A05/MF A01 - OSTI; GPO Dep.

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  • Other Information: Thesis (Ph.D.)

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  • Other: DE91001791
  • Report No.: LBL-28624
  • Grant Number: AC03-76SF00098
  • DOI: 10.2172/6374468 | External Link
  • Office of Scientific & Technical Information Report Number: 6374468
  • Archival Resource Key: ark:/67531/metadc1209134

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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Creation Date

  • November 1, 1989

Added to The UNT Digital Library

  • July 5, 2018, 11:11 p.m.

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  • Oct. 30, 2018, 12:25 p.m.

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Kong, T. Reconstitution of halorhodopsin, report, November 1, 1989; California. (digital.library.unt.edu/ark:/67531/metadc1209134/: accessed November 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.