Direct PCR amplification of DNA from human bloodstains, saliva, and touch samples collected with microFLOQ® swabs

PDF Version Also Available for Download.

Description

This article investigates the efficacy of direct amplification of DNA from bloodstains, saliva stains, and touch samples using microFLOQ® Direct swabs and the GlobalFiler™ Express system (Thermo Fisher Scientific, Waltham, MA).

Physical Description

8 p.

Creation Information

Ambers, Angie D.; Wiley, Rachel; Novroski, Nicole & Budowle, Bruce October 26, 2017.

Context

This article is part of the collection entitled: UNT Scholarly Works and was provided by UNT College of Arts and Sciences to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 16 times . More information about this article can be viewed below.

Who

People and organizations associated with either the creation of this article or its content.

Authors

  • Ambers, Angie D. University of North Texas; University of North Texas Health Science Center
  • Wiley, Rachel University of North Texas Health Science Center
  • Novroski, Nicole University of North Texas Health Science Center
  • Budowle, Bruce University of North Texas Health Science Center; King Abdulaziz University

Publisher

Provided By

UNT College of Arts and Sciences

The UNT College of Arts and Sciences educates students in traditional liberal arts, performing arts, sciences, professional, and technical academic programs. In addition to its departments, the college includes academic centers, institutes, programs, and offices providing diverse courses of study.

Contact Us

What

Descriptive information to help identify this article. Follow the links below to find similar items on the Digital Library.

Degree Information

Description

This article investigates the efficacy of direct amplification of DNA from bloodstains, saliva stains, and touch samples using microFLOQ® Direct swabs and the GlobalFiler™ Express system (Thermo Fisher Scientific, Waltham, MA).

Physical Description

8 p.

Notes

Abstract: Previous studies have shown that nylon flocked swabs outperform traditional fiber swabs in DNA recovery due to
their innovative design and lack of internal absorbent core to entrap cellular materials. The microFLOQ® Direct
swab, a miniaturized version of the 4N6 FLOQSwab®, has a small swab head that is treated with a lysing agent
which allows for direct amplification and DNA profiling from sample collection to final result in less than two
hours. Additionally, the microFLOQ® system subsamples only a minute portion of a stain and preserves the vast
majority of the sample for subsequent testing or re-analysis, if desired. The efficacy of direct amplification of
DNA from dilute bloodstains, saliva stains, and touch samples was evaluated using microFLOQ® Direct swabs and
the GlobalFiler™ Express system. Comparisons were made to traditional methods to assess the robustness of this
alternate workflow. Controlled studies with 1:19 and 1:99 dilutions of bloodstains and saliva stains consistently
yielded higher STR peak heights than standard methods with 1 ng input DNA from the same samples. Touch
samples from common items yielded single source and mixed profiles that were consistent with primary users of
the objects. With this novel methodology/workflow, no sample loss occurs and therefore more template DNA is
available during amplification. This approach may have important implications for analysis of low quantity and/
or degraded samples that plague forensic casework.

Source

  • Forensic Science International: Genetics, 2018. Amsterdam, The Netherlands: Elsevier

Language

Item Type

Identifier

Unique identifying numbers for this article in the Digital Library or other systems.

Publication Information

  • Publication Title: Forensic Science International: Genetics
  • Volume: 32
  • Pages: 80-87
  • Peer Reviewed: Yes

Collections

This article is part of the following collection of related materials.

UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

What responsibilities do I have when using this article?

When

Dates and time periods associated with this article.

Creation Date

  • October 26, 2017

Submitted Date

  • June 8, 2017

Accepted Date

  • October 25, 2017

Added to The UNT Digital Library

  • May 16, 2018, 2:54 p.m.

Usage Statistics

When was this article last used?

Yesterday: 0
Past 30 days: 3
Total Uses: 16

Interact With This Article

Here are some suggestions for what to do next.

Start Reading

PDF Version Also Available for Download.

International Image Interoperability Framework

IIF Logo

We support the IIIF Presentation API

Ambers, Angie D.; Wiley, Rachel; Novroski, Nicole & Budowle, Bruce. Direct PCR amplification of DNA from human bloodstains, saliva, and touch samples collected with microFLOQ® swabs, article, October 26, 2017; Amsterdam, The Netherlands. (digital.library.unt.edu/ark:/67531/metadc1152218/: accessed September 25, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.