Alterations in Fatty Acid Amide Hydrolase (Faah) Transcript Levels and Activity Lead to Changes in the Abiotic Stress Susceptibility of Arabidopsis Thaliana Page: 2
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Gonzalez, Gabriel. Alterations in Fatty Acid Amide Hydrolase (FAAH)
Transcript Levels and Activity Lead to Changqes in the Abiotic Stress
Susceptibility of Arabidopsis thaliana. Master of Science (Biochemistry and
Molecular Biology), May 2012, 90 pp., 2 tables, 20 illustrations, 73 references.
N-Acylethanolamines (NAEs) are a class of bioactive lipids, and FAAH is
one of the enzymes responsible for degrading NAEs in both plants and animals.
In plants, FAAH appears to be closely associated with ABA, a phytohormone
which has long been associated with plant stress responses, since the
overexpression of FAAH in Arabidopsis results in ABA hypersensitivity.
Therefore, it is reasonable to speculate that alterations in FAAH transcript levels
will result in altered stress responses in plants. To investigate this hypothesis
experiments were carried out in which wild type (WT), FAAH-overexpressing
(OE), and T-DNA insertional FAAH knockouts of Arabidopsis (faah) were grown
in MS media under stress conditions. The stress conditions tested included
chilling stress, heavy metal stress induced by cadmium or copper, nutrient
limitations induced by low phosphorus or low nitrogen, salt stress induced with
NaCI, and osmotic stress induced with mannitol. The OE plants were
consistently hypersensitive to all stress conditions in relation to wild type plants.
Inactive FAAH overexpressors did not have the hypersensitivity to the salt and
osmotic stress of the active OE plants and were instead tolerant to these
stresses. FAAH2 (faah2) knockouts and FAAH 1 and 2 double knockouts (faah
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Gonzalez, Gabriel. Alterations in Fatty Acid Amide Hydrolase (Faah) Transcript Levels and Activity Lead to Changes in the Abiotic Stress Susceptibility of Arabidopsis Thaliana, thesis, May 2012; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc115085/m1/2/: accessed November 15, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .