Comparing biological markers of Alzheimer’s disease across blood fraction and platforms: Comparing apples to oranges

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This article investigates the comparability of potential Alzheimer's disease (AD) biomarkers across blood fractions and assay platforms.

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8 p.

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O'Bryant, Sid E.; Lista, Simone; Rissman, Robert A.; Edwards, Melissa; Zhang, Fan; Hall, James R. et al. December 29, 2015.

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Description

This article investigates the comparability of potential Alzheimer's disease (AD) biomarkers across blood fractions and assay platforms.

Physical Description

8 p.

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Abstract
Introduction

This study investigated the comparability of potential Alzheimer's disease (AD) biomarkers across blood fractions and assay platforms.
Methods

Nonfasting serum and plasma samples from 300 participants (150 AD patients and 150 controls) were analyzed. Proteomic markers were obtained via electrochemiluminescence or Luminex technology. Comparisons were conducted via Pearson correlations. The relative importance of proteins within an AD diagnostic profile was examined using random forest importance plots.
Results

On the Meso Scale Discovery multiplex platform, 10 of the 21 markers shared >50% of the variance across blood fractions (serum amyloid A R2 = 0.99, interleukin (IL)10 R2 = 0.95, fatty acid-binding protein (FABP) R2 = 0.94, I309 R2 = 0.94, IL-5 R2 = 0.94, IL-6 R2 = 0.94, eotaxin3 R2 = 0.91, IL-18 R2 = 0.87, soluble tumor necrosis factor receptor 1 R2 = 0.85, and pancreatic polypeptide R2 = 0.81). When examining protein concentrations across platforms, only five markers shared >50% of the variance (beta 2 microglobulin R2 = 0.92, IL-18 R2 = 0.80, factor VII R2 = 0.78, CRP R2 = 0.74, and FABP R2 = 0.70).
Discussion

The current findings highlight the importance of considering blood fractions and assay platforms when searching for AD relevant biomarkers.

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  • Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring, 2016. Amsterdam, The Netherlands: Elsevier

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  • Publication Title: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
  • Volume: 3
  • Pages: 27-34
  • Peer Reviewed: Yes

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UNT Scholarly Works

Materials from the UNT community's research, creative, and scholarly activities and UNT's Open Access Repository. Access to some items in this collection may be restricted.

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  • December 29, 2015

Added to The UNT Digital Library

  • May 1, 2018, 12:41 a.m.

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O'Bryant, Sid E.; Lista, Simone; Rissman, Robert A.; Edwards, Melissa; Zhang, Fan; Hall, James R. et al. Comparing biological markers of Alzheimer’s disease across blood fraction and platforms: Comparing apples to oranges, article, December 29, 2015; Amsterdam, The Netherlands. (digital.library.unt.edu/ark:/67531/metadc1132747/: accessed June 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.