''Aged'' (dense) circulating red cells contain normal concentrations of ATP

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A newly-developed technique for determination of the ATP content of individual red cells to the densest, and hence presumably the oldest, cells from normal human blood was applied. It was found that these cells contain normal concentrations of ATP, although the net content of ATP is decreased. The essence of the technique is suspension of red cells in autologous plasma containing luciferin and luciferase, lysis of the cells with a pulse from a laser, and counting of the photoemissions resulting from reaction of the released ATP with the luciferase. These data appear to disprove the otherwise plausible hypothesis of Lichtman ... continued below

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Pages: 7

Creation Information

Kirkpatrick, F.H.; Muhs, A.G.; Kostuk, R.K. & Gabel, C.W. January 1, 1978.

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Description

A newly-developed technique for determination of the ATP content of individual red cells to the densest, and hence presumably the oldest, cells from normal human blood was applied. It was found that these cells contain normal concentrations of ATP, although the net content of ATP is decreased. The essence of the technique is suspension of red cells in autologous plasma containing luciferin and luciferase, lysis of the cells with a pulse from a laser, and counting of the photoemissions resulting from reaction of the released ATP with the luciferase. These data appear to disprove the otherwise plausible hypothesis of Lichtman that red cells decline exponentially in ATP content as they age, by one of the suggested tests of this hypothesis. The data suggest an alternative hypothesis: red cells maintain an approximately constant concentration of ATP as they age, and red cell destruction is caused by factors other than cellular ATP.

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Pages: 7

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Dep. NTIS, PC A02/MF A01.

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  • Report No.: UR-3490-1543
  • Grant Number: EY-76-C-02-3490
  • DOI: 10.2172/6230591 | External Link
  • Office of Scientific & Technical Information Report Number: 6230591
  • Archival Resource Key: ark:/67531/metadc1113132

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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Creation Date

  • January 1, 1978

Added to The UNT Digital Library

  • Feb. 22, 2018, 7:45 p.m.

Description Last Updated

  • March 20, 2018, 5:45 p.m.

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Kirkpatrick, F.H.; Muhs, A.G.; Kostuk, R.K. & Gabel, C.W. ''Aged'' (dense) circulating red cells contain normal concentrations of ATP, report, January 1, 1978; United States. (digital.library.unt.edu/ark:/67531/metadc1113132/: accessed September 26, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.