Biotechnology and genetic optimization of fast-growing hardwoods

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Description

A biotechnology research program was initiated to develop new clones of fast-growing Populus clones resistant to the herbicide glyphosate and resistant to the leaf-spot and canker disease caused by the fungus Septoria musiva. Glyphosate-resistant callus was selected from stem segments cultured in vitro on media supplemented with the herbicide. Plants were regenerated from the glyphosate-resistant callus tissue. A portion of plants reverted to a glyphosate susceptible phenotype during organogenesis. A biologically active filtrate was prepared from S. musiva and influenced fresh weight of Populus callus tissue. Disease-resistant plants were produced through somaclonal variation when shoots developed on stem internodes cultured ... continued below

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Pages: (91 p)

Creation Information

Garton, S.; Syrkin-Wurtele, E.; Griffiths, H.; Schell, J.; Van Camp, L. & Bulka, K. (NPI, Salt Lake City, UT (United States)) February 1, 1991.

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Description

A biotechnology research program was initiated to develop new clones of fast-growing Populus clones resistant to the herbicide glyphosate and resistant to the leaf-spot and canker disease caused by the fungus Septoria musiva. Glyphosate-resistant callus was selected from stem segments cultured in vitro on media supplemented with the herbicide. Plants were regenerated from the glyphosate-resistant callus tissue. A portion of plants reverted to a glyphosate susceptible phenotype during organogenesis. A biologically active filtrate was prepared from S. musiva and influenced fresh weight of Populus callus tissue. Disease-resistant plants were produced through somaclonal variation when shoots developed on stem internodes cultured in vitro. Plantlets were screened for disease symptoms after spraying with a suspension of fungal spores. A frequency of 0.83 percent variant production was observed. Genetically engineered plants were produced after treatment of plant tissue with Agrobacterium tumefasciens strains carrying plasmid genes for antibiotic resistance. Transformers were selected on media enriched with the antibiotic, kanamycin. Presence of foreign DNA was confirmed by Southern blot analysis. Protoplasts of popular were produced but did not regenerate into plant organs. 145 refs., 12 figs., 36 tabs.

Physical Description

Pages: (91 p)

Notes

OSTI; New York State Energy Research and Development Authority, Two Rockefeller Plaza, Albany, NY 12223

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  • Other: TI92002650
  • Report No.: NYSERDA-91-8
  • Grant Number: None
  • DOI: 10.2172/6199269 | External Link
  • Office of Scientific & Technical Information Report Number: 6199269
  • Archival Resource Key: ark:/67531/metadc1107808

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • February 1, 1991

Added to The UNT Digital Library

  • Feb. 22, 2018, 7:45 p.m.

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Garton, S.; Syrkin-Wurtele, E.; Griffiths, H.; Schell, J.; Van Camp, L. & Bulka, K. (NPI, Salt Lake City, UT (United States)). Biotechnology and genetic optimization of fast-growing hardwoods, report, February 1, 1991; United States. (https://digital.library.unt.edu/ark:/67531/metadc1107808/: accessed March 21, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.