Microsomal lipid peroxidation as a mechanism of cellular damage. [Dissertation]

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The NADPH/iron-dependent peroxidation of lipids in rat liver microsomes was found to be dependent on the presence of free ferrous ion and maintains iron in the reduced Fe/sup 2 +/ state. Chelation of iron by EDTA inhibited peroxidation. Addition of iron, after preincubation of microsomes in the absence of iron, did not enhance the rate of peroxidation suggesting that iron acts by initiating peroxidative decomposition of membrane lipids rather than by catalyzing the breakdown of pre-formed hydroperoxides. Liposomes also underwent peroxidation in the presence of ferrous iron at a rate comparable to intact microsomes and was stimulated by ascorbate. Carbon ... continued below

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Pages: 142

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Kornbrust, D.J. January 1, 1979.

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Description

The NADPH/iron-dependent peroxidation of lipids in rat liver microsomes was found to be dependent on the presence of free ferrous ion and maintains iron in the reduced Fe/sup 2 +/ state. Chelation of iron by EDTA inhibited peroxidation. Addition of iron, after preincubation of microsomes in the absence of iron, did not enhance the rate of peroxidation suggesting that iron acts by initiating peroxidative decomposition of membrane lipids rather than by catalyzing the breakdown of pre-formed hydroperoxides. Liposomes also underwent peroxidation in the presence of ferrous iron at a rate comparable to intact microsomes and was stimulated by ascorbate. Carbon tetrachloride initiated lipid peroxidation in the absence of free metal ions. Rates of in vitro lipid peroxidation of microsomes and homogenates were found to vary widely between different tissues and species. The effects of paraquat on lipid peroxidation was also studied. (DC)

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Pages: 142

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Dep. NTIS, PC A07/MF A01.

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  • Other Information: Thesis

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  • Report No.: UR-3490-1765
  • Grant Number: EY-76-C-02-3490
  • DOI: 10.2172/5825958 | External Link
  • Office of Scientific & Technical Information Report Number: 5825958
  • Archival Resource Key: ark:/67531/metadc1096659

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Office of Scientific & Technical Information Technical Reports

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Creation Date

  • January 1, 1979

Added to The UNT Digital Library

  • Feb. 18, 2018, 3:59 p.m.

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  • March 20, 2018, 6:02 p.m.

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Kornbrust, D.J. Microsomal lipid peroxidation as a mechanism of cellular damage. [Dissertation], report, January 1, 1979; United States. (digital.library.unt.edu/ark:/67531/metadc1096659/: accessed May 22, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.