Determining biological fine structure by differential absorption of soft x-rays. [Ultrastructural studies of nerve fibers using scanning electron microscopy without need for staining or osmication]

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The use of soft x-ray contact microscopy in examining histochemically treated human tissue embedded in plastic and exposed as unstained thin sections is demonstrated. When our preliminary data revealed that we could clearly image not only the histochemical reaction product, but the unstained biological fine structure of the surrounding tissues, we decided to test our hypothesis further and see if we could image unstained biological molecular aggregates as well. For this part of the investigation, we chose to examine hydrated proteoglycan aggregates. Proteoglycans are an essential component of the organic matrix of cartilage, and play a primary role in the ... continued below

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Pages: 23

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Panessa-Warren, B.J. & Warren, J.B. June 1, 1979.

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Description

The use of soft x-ray contact microscopy in examining histochemically treated human tissue embedded in plastic and exposed as unstained thin sections is demonstrated. When our preliminary data revealed that we could clearly image not only the histochemical reaction product, but the unstained biological fine structure of the surrounding tissues, we decided to test our hypothesis further and see if we could image unstained biological molecular aggregates as well. For this part of the investigation, we chose to examine hydrated proteoglycan aggregates. Proteoglycans are an essential component of the organic matrix of cartilage, and play a primary role in the retention and maintenance of extracellular water. To avoid any artifacts due to the introduction of exogeneous materials, and examine the proteoglycan aggregates in their hydrated, natural configuration, we made contact x-ray images of isolated proteoglycan aggregates in water.

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Pages: 23

Notes

Dep. NTIS, PC A02/MF A01.

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  • Conference on ultrasoft X-ray microscopy, New York, NY, USA, 13 Jun 1979

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  • Report No.: BNL-26282
  • Report No.: CONF-790674-3
  • Grant Number: EY-76-C-02-0016
  • Office of Scientific & Technical Information Report Number: 5934556
  • Archival Resource Key: ark:/67531/metadc1095280

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Office of Scientific & Technical Information Technical Reports

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  • June 1, 1979

Added to The UNT Digital Library

  • Feb. 18, 2018, 3:59 p.m.

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  • March 20, 2018, 8:42 p.m.

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Panessa-Warren, B.J. & Warren, J.B. Determining biological fine structure by differential absorption of soft x-rays. [Ultrastructural studies of nerve fibers using scanning electron microscopy without need for staining or osmication], article, June 1, 1979; United States. (digital.library.unt.edu/ark:/67531/metadc1095280/: accessed September 23, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.