The application of XPS to the study of MIC Page: 4 of 15

Desulfovibrio sp. in a modified Postgate’s medium C was examined. The
ofTsSSS.dc study of microbial activity and the effects of aUoyrng elements and
thermo-mechanical treatments on the MIC resistance of stainless stee s.
EXPERIMENTAL
chemical and spectroscopic means.
%
Microbiology
Culture Desulfovibrio sp.; isolated from a leachate sample that had the
ing^chtuacteristics: reduces sulfate, desulfoviridin positive, gram negative, vibrioid
^M^um^MtSfiS'PosTgTTTmStTn C consisting of (g/»i1
use of lactic add instead of sodium lactate.) The medium contained 1800 gM sulfate
and 900 pM lactic acid. The medium was pre-reduced, by boiiing^d p^gmg^wi
N, and dispensed in 60 ml serum bottles in an anaerobic glove box filledwith 95%
N2 and 5% Hj, as described previously.[24] The bottles were capped with butyl-ru^
ber stoppers, crimped and autoclaved at 121°C at 20 psi for min. p
the medium after sterilization was 7.4. , , ,
Metals. 0.2 mM of ferric chloride (FeCl3*6H20), sodium molybdate
(Na^MoOi®2H20), chromic chloride (CrCl3*6H20) and mckelous chlon
(NiCl2*6H20) were separately added to sterile medium. The solutions containing
the metal ions were added to the sterile medium after filtration through a 0.22 nm
filter. The final concentrations of metal ions in the medium are Z A
The pH of the medium after the addition of the metal ions was readjusted to 7.4. A
2 5% v/v inoculum of a 2-day-old culture was used in the experiments.
1 anH Chemical Analysis, At the end of the incubation penod,jhe
foUowing~were measured: (1) lactic add consumption, (2) productonto
TlcUPornP(S«'F^el’^d'Tab’ll'TTuta^mlts.) TEty »uld no* be used as
attach^! tol 22 gauge needle. The culture samples were transferred into40 ml acid
washed centrifuge tubes and centrifuged at 10,000 rpm for 15 minutes. Th
supernate was decanted, the pH was determined, the sample was altered throug
0 22 urn filter, and analyzed for metals, sulfate, and organic acids. The cell-pellets
(bacterial cells and sulfide precipitates) were placed in a desiccator and allowed to
dry under anoxic conditions for two days. The uninoculated samples, which
served as experimental controls, were also analyzed for pH, metals, sulfate and
178/3
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Kearns, J.R. (Allegheny Ludlum Corp., Brackenridge, PA (United States). Technical Center); Clayton, C.R.; Halada, G.P. (State Univ. of New York, Stony Brook, NY (United States). Dept. of Materials Science); Gillow, J.B. & Francis, A.J. (Brookhaven National Lab., Upton, NY (United States)). The application of XPS to the study of MIC, article, January 1, 1992; Upton, New York. (https://digital.library.unt.edu/ark:/67531/metadc1094209/m1/4/ocr/: accessed April 20, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.

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