Studies of membrane structure by freeze-etching. Progress report

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The researchers devised a rapid procedure for measuring covalent bond breakage in a simple model system to determine the extent to which glycophorin was cleaved when the frozen vesicles were fractured. Evidence showed that no covalent bonds are broken during the fracture procedure. Membrane splitting was shown to be quantified under special circumstances when erythrocyte membranes are appressed to a polylysine-coated surface. Results show that freeze fracture at temperatures below that of liquid nitrogen results in the appearance of considerable detail on E faces of purple membranes. Detail seems to improve as the temperature is lowered, suggesting that the smooth ... continued below

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Pages: 9

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Branton, D January 1, 1979.

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Description

The researchers devised a rapid procedure for measuring covalent bond breakage in a simple model system to determine the extent to which glycophorin was cleaved when the frozen vesicles were fractured. Evidence showed that no covalent bonds are broken during the fracture procedure. Membrane splitting was shown to be quantified under special circumstances when erythrocyte membranes are appressed to a polylysine-coated surface. Results show that freeze fracture at temperatures below that of liquid nitrogen results in the appearance of considerable detail on E faces of purple membranes. Detail seems to improve as the temperature is lowered, suggesting that the smooth E faces in conventional freeze fractures may be a result of plastic deformation and flow of membrane components. Results show that ultra-low temperature freeze-fracturing can reduce plastic distortion and, particularly when used in conjunction with rotary replication, should improve our ability to discern small labels attached to transmembrane proteins. (PCS)

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Pages: 9

Notes

Dep. NTIS, PC A02/MF A01.

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  • Report No.: DOE/EV/0243-1
  • Grant Number: EY-76-S-02-2423
  • DOI: 10.2172/5578792 | External Link
  • Office of Scientific & Technical Information Report Number: 5578792
  • Archival Resource Key: ark:/67531/metadc1093894

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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Creation Date

  • January 1, 1979

Added to The UNT Digital Library

  • Feb. 10, 2018, 10:06 p.m.

Description Last Updated

  • April 17, 2018, 1:27 p.m.

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Branton, D. Studies of membrane structure by freeze-etching. Progress report, report, January 1, 1979; United States. (digital.library.unt.edu/ark:/67531/metadc1093894/: accessed October 21, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.