Molecular biological enhancement of coal desulfurization: Cloning and expression of the sulfoxide/sulfone/sulfonate/sulfate genes in Pseudomonads and Thiobacillae

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This department of energy-sponsored mission-oriented research project currently has three emphases. They are: (1) determining the kinetics of monohydroxybiphenyl (OH-BP) production in batch culture by N1-36, an unidentified soil isolate, when the organism is presented with dibenzothiophene (DBT) or dibenzosulfone (DBTO{sub 2}); (2) establishing reliable methods for physically characterizing R68.45, a broad host range plasmid; and (3) attempting to elicit a physiologically consistent and genetically stable ability of some gram negative soil isolates to convert DBT to o,o{prime}-biphenol. Eachh of these goals has subsidiary components. For example, in addition to establishing kinetics of formation of OH-BP by N1-36, analyses have … continued below

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12 pages

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Krawiec, S. November 9, 1991.

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This department of energy-sponsored mission-oriented research project currently has three emphases. They are: (1) determining the kinetics of monohydroxybiphenyl (OH-BP) production in batch culture by N1-36, an unidentified soil isolate, when the organism is presented with dibenzothiophene (DBT) or dibenzosulfone (DBTO{sub 2}); (2) establishing reliable methods for physically characterizing R68.45, a broad host range plasmid; and (3) attempting to elicit a physiologically consistent and genetically stable ability of some gram negative soil isolates to convert DBT to o,o{prime}-biphenol. Eachh of these goals has subsidiary components. For example, in addition to establishing kinetics of formation of OH-BP by N1-36, analyses have been or will be performed to determine whether the catalytic activity can be achieved with irradiated cells, spheroplasts, and cell extracts. This report presents information on progress towards fulfilling both the principal goals listed above and some relevant ancillary activities.

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12 pages

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OSTI; NTIS; GPO Dep.

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  • November 9, 1991

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Thumbnail image of item number 1 in: 'Molecular biological enhancement of coal desulfurization: Cloning and expression of the sulfoxide/sulfone/sulfonate/sulfate genes in Pseudomonads and Thiobacillae'.
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Krawiec, S. Molecular biological enhancement of coal desulfurization: Cloning and expression of the sulfoxide/sulfone/sulfonate/sulfate genes in Pseudomonads and Thiobacillae, report, November 9, 1991; United States. (https://digital.library.unt.edu/ark:/67531/metadc1093387/: accessed April 18, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.

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