Somatic cell and molecular genetics approach to DNA repair and mutagenesis

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In the CHO cell line, UV-sensitive mutants representing five genetic complementation groups have been identified. Mutants from each of these groups were shown to be defective in performing the incision step of repair after exposure to UV. The large number of complementation groups of xeroderma pigmentosa mutations has raised the question whether these groups all correspond to single gene loci. The same issue applies to the 5 groups of UV-sensitive CHO mutants. One approach toward answering this question is to localize in the human karyotype the genes that complement the defects in the CHO mutants. Thus, by making CHO/human cell ... continued below

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Pages: 10

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Thompson, L.H. June 14, 1985.

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Description

In the CHO cell line, UV-sensitive mutants representing five genetic complementation groups have been identified. Mutants from each of these groups were shown to be defective in performing the incision step of repair after exposure to UV. The large number of complementation groups of xeroderma pigmentosa mutations has raised the question whether these groups all correspond to single gene loci. The same issue applies to the 5 groups of UV-sensitive CHO mutants. One approach toward answering this question is to localize in the human karyotype the genes that complement the defects in the CHO mutants. Thus, by making CHO/human cell hybrids under the appropriate selective conditions, we have begun to map each of the complementing human genes. The mutation in strain UV20 (Group 2) was complemented by human chromosome 19. Preliminary evidence suggests that UV5 may also be complemented by human chromosome 19 while each of the other 3 groups involves a different human chromosome. Somewhat surprisingly, mutant EM9 is also complemented by a gene on chromosome 19.

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Pages: 10

Notes

NTIS, PC A02/MF A01.

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  • 4. international conference on environmental mutagens, Stockholm, Sweden, 24 Jun 1985

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  • Other: DE85014181
  • Report No.: UCRL-92834
  • Report No.: CONF-8506137-4
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 5673479
  • Archival Resource Key: ark:/67531/metadc1092202

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • June 14, 1985

Added to The UNT Digital Library

  • Feb. 10, 2018, 10:06 p.m.

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  • April 27, 2018, 2:11 p.m.

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Thompson, L.H. Somatic cell and molecular genetics approach to DNA repair and mutagenesis, article, June 14, 1985; [Livermore,] California. (digital.library.unt.edu/ark:/67531/metadc1092202/: accessed December 14, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.