Approaches to the preservation of human granulocytes by freezing

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Because of its simplicity, the FDA assay can be used effectively as a screening test to eliminate procedures and treatments that are damaging to cells. In this context, a number of conclusions can be drawn from the data presented: (1) Exposure to 1 and 2 M glycerol at room temperature damages human granulocytes in a few minutes. Reducing the exposure temperature to 0/sup 0/C reduces the amount of injury substantially. (2) Human granulocytes respond to freezing and thawing in a manner typical of many mammalian cells in that they exhibit a maximum in survival at an optimum cooling rate slightly ... continued below

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Pages: 13

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Frim, J & Mazur, P January 1, 1979.

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Description

Because of its simplicity, the FDA assay can be used effectively as a screening test to eliminate procedures and treatments that are damaging to cells. In this context, a number of conclusions can be drawn from the data presented: (1) Exposure to 1 and 2 M glycerol at room temperature damages human granulocytes in a few minutes. Reducing the exposure temperature to 0/sup 0/C reduces the amount of injury substantially. (2) Human granulocytes respond to freezing and thawing in a manner typical of many mammalian cells in that they exhibit a maximum in survival at an optimum cooling rate slightly above 1/sup 0/C/min when combined with rapid warming. The use of rapid warming and a high (2 M) concentration of glycerol reduces the dependence of survival on cooling rate by broadening the range of rates over which survival is relatively high. (3) Human granulocytes show some sensitivity to dilution stresses since survival depends somewhat on the concentration of glycerol used and the severity of the dilution procedure. The reasons for the sharp decrease in cell viability following incubation of frozen-thawed granulocytes at 37/sup 0/C are not known. One possibility is that the phosphate buffered saline suspending medium used is not suitable for incubation at 37/sup 0/C. A second possibility is that some cell injury is not expressed at 0/sup 0/C and remains undetected by the FDA assay until the cells are incubated at 37/sup 0/C. There is also the possibility that lysosomal enzymes released by a few damaged cells in a sample will cause additional damage in other cells at 37/sup 0/C.

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Pages: 13

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Dep. NTIS, PC A02/MF A01.

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  • Symposium on storage and preservation of granulocytes, Atlanta, GA, USA, 30 Sep 1979

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  • Report No.: CONF-7909135-1
  • Grant Number: W-7405-ENG-26
  • Office of Scientific & Technical Information Report Number: 5501258
  • Archival Resource Key: ark:/67531/metadc1092173

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • January 1, 1979

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  • Feb. 10, 2018, 10:06 p.m.

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  • April 16, 2018, 6:40 p.m.

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Frim, J & Mazur, P. Approaches to the preservation of human granulocytes by freezing, article, January 1, 1979; Tennessee. (digital.library.unt.edu/ark:/67531/metadc1092173/: accessed September 24, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.