Metabolism of Hydrocortisone by X-Irradiated Rat Liver Tissue as Determined by the Porter-Silber Chromagen Method Page: 18
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media by livers removed at various time intervals following
irradiation was plotted and compared with the rates exhibited
by unirradiated livers.
Figure 2 demonstrates the effect of a dosage of 428r
whole body X-irradiation on hydrocortisone metabolism. The
livers were removed and analyzed for their ability to meta-
bolize hydrocortisone at four hours, twenty-four hours and
two weeks after X-lrradiation• Livers removed 4 hours after
irradiation metabolized 30.6 ug of hydrocortisone per 100 rag
of tissue per hour• A slightly less amount of hydrocortisone
was metabolized by liver removed 24 hours following irradi-
ation of 428r♦ At the 2-week Interval following irradiation
only 29.2 ug of hydrocortisone were metabolized per 100 mg. of
tissue per hour• This dosage is considered to be an LD50 dose•
At a dosage of 856r, as shown graphically in Figure 5,
there is seen a similar pattern of decrease in the ability
of X-irradiated liver to metabolize hydrocortisone• At the
4-hour period the liver was able to metabolize 32.8 ug of
hydrocortisone per 100 mg. of tissue per hour. At the 24 hour
and 2 week periods the decrease in metabolism was greater as
only 30 ug. and 30.5 ug. of steroid was metabolized per 100 rag.
of tissue per hour. This dosage of 85(3r is lethal for 100 per
cent of the cases in 50 days. The variation in metabolism at
the different time intervals following irradiation with dosages
of 428r and 856r is not thought to be highly significant.
Figure 4 shows the results obtained at the 1712r dosage
level. The most significant decrease in ability of X-irradlafced
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Pryor, Nan Webb. Metabolism of Hydrocortisone by X-Irradiated Rat Liver Tissue as Determined by the Porter-Silber Chromagen Method, thesis, August 1959; Denton, Texas. (https://digital.library.unt.edu/ark:/67531/metadc108062/m1/23/: accessed March 21, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; .