u
fh@ amount of hydrocortisone fvaa the  #dlii in
micrograms per 100 mg* of tissue per hour was calculated from
the full-wing formula i
(Sj. XV)- (8* X f)
 S J s  x 100
whey#«
Sj, - Concentre felon ©f st eroid In sample with a® tissue *
S|r of staroid la sample with tissue.
? - Ml. of chloroform solution used in eactrsfition (IS ml*)•
X - Kg* ©f tissue used In sample (§00 mg.)•
Oaing this foraula the ajsount of hydrocortlaone removed
fro® the media was determined for each test sample and recorded.
A mean fa la® of the teat samples «as determined frost rata in
the same group 1 * e., rata receiving the same dosage of
X-trradlatlesi sad having their livera removed at the same
tia® following the irradiation*