Metabolism of Hydrocortisone by X-Irradiated Rat Liver Tissue as Determined by the Porter-Silber Chromagen Method Page: 14
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fh@ amount of hydrocortisone fvaa the #dlii in
micrograms per 100 mg* of tissue per hour was calculated from
the full-wing formula i
(Sj. XV)- (8* X f)
S J s x 100
Sj, - Concentre felon ©f st eroid In sample with a® tissue *
S|r of staroid la sample with tissue.
? - Ml. of chloroform solution used in eactrsfition (IS ml*)•
X - Kg* ©f tissue used In sample (§00 mg.)•
Oaing this foraula the ajsount of hydrocortlaone removed
fro® the media was determined for each test sample and recorded.
A mean fa la® of the teat samples «as determined frost rata in
the same group 1 * e., rata receiving the same dosage of
X-trradlatlesi sad having their livera removed at the same
tia® following the irradiation*
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Pryor, Nan Webb. Metabolism of Hydrocortisone by X-Irradiated Rat Liver Tissue as Determined by the Porter-Silber Chromagen Method, thesis, August 1959; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc108062/m1/19/: accessed February 17, 2019), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .