Metabolism of Hydrocortisone by X-Irradiated Rat Liver Tissue as Determined by the Porter-Silber Chromagen Method Page: 12
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tm 3© *inate*. A.fto* thirty minutes the taboo were veaovod
ipm the w te* bath and held ondea? o©ld running mtw f«
spp*flacl&*tely th*eo minutes to *ttp tho ®#Jt« ir# «tio « fta®
speotrophetOKotey was set using the mmgmt bleak as zero
opt let 1 density* the option! density of eeoh teat tube ana
its respective blank tabe wo® taken and «••«*€*&• The Msohlne
was eheoleed often with the reagent blank for drifting#
A standsrd optlosl density carve for hydrocortisone w««-
made by finding the optical densities of tecwm oenoenfiratleiMi
of the atorold and plotting thou «n a graph. ft*# standard
*ur * 1ft shown t Figaro 1 sad In shown to b© a straight lino
«fp with the optical density varying la direct proportion
to th@ mmmtmtlm of sterol** this o s?ir« ii specific for
hydrocortisone * Other corticosteroids mm also fount -to
give « it flight line wrnm with the optical densities plotted
against the concentrations, bmfe tho slope of the curve ilf*
fered with oaoh, steroid tried* the known concentrations were
stffte by preparing a series of ton dll&tlcns*
the concentration of tho anlmowa topple wss determined
by subtracting tho optical density of tho blank (Sot II) frout
tho optical density of tho test sample (Set I). fhls procedure
corrects for any ohr wattle interference from * reaction be-
tween the reagents* the yesmltsmt optica1 density was then
used to find tbo concentration of tho imtaown sample by aeans
of tho standard' opt tool density curve for hydrocortisone#
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Pryor, Nan Webb. Metabolism of Hydrocortisone by X-Irradiated Rat Liver Tissue as Determined by the Porter-Silber Chromagen Method, thesis, August 1959; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc108062/m1/17/: accessed February 21, 2019), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .