m
manna*. fli® original solution and fcb© two m*biag« «•«  #©«-
tela®# to make   total of itwn si* «f solution.
fanral.# of chloroform «*** added ta tha jsSjetwa of
inai solation of buffer isA ataroid and the few© waskingsj* fha
tuba* *«« ©appad and giwn ®& sfaak## bj brad  p.iaead In feist
aba tear foa? $ aimttoa at ISO ppa ted than given SO nova abates
band* Tba tuba* war® than oantMfttga# foa* 5 isin&fcaa *1
lOOO <vpn* ■ fhe ®h%m®£®m (batten) Imm wifta a
-aail to%lb and pal- in a' claan tuba# . &ffc«r tbe first
«t efci«. of eti Iodoform w « raaoirad, e *1* ©f fresh ©hloro-
form were added to tb ' origin® 1, aolmiiran .ami na-abiagai -fetg a
second *xtv«etie«u< Again the efeler&fevat ls.3w.waa rewnred
after thorough adbiiag moA «d#ai. fee the £!*«%' - Tbe
tube* eonfcfiining efalorofona we* @ toft tightly: ©appad «• anob
M poaaibla I© prevent «v*pe* tiw of chloroform wbi#ii might
elte* the eeneeatretien of steroid, the tube* containing feto©
ohl©r©foi«st amtraotiona were taveyfee* itee®. tlaee to isssare
tfsjal, dlfttvlbaticni of the steroid •
'' tee si* of ®itlm®tmm aolutian wee veueved and placed in -
a tuba tm evaporation* 4 dtipiloat® simple of ehlovtifens waa
placed in toolbar trnbe to be aaed as # bettolegeu* blank# fhe
twe aata of uaeepped tobee, Sat I and Safe II, eaofa oontaiatag
1 *1# of *hl4eof«Mr ttolukien* wey* pleeed in a d®*i#fst#* whloh
mmn in a «#l®f bath at 6® degseee Centigrade*' She 6e«ls*ftt«*,
waa oonnactad to. the mmma pimp tteromgto a dry ice toafth de-
signed to ®«#e #e tha eblevefora and thus present reeetien