Metabolism of Hydrocortisone by X-Irradiated Rat Liver Tissue as Determined by the Porter-Silber Chromagen Method Page: 10
This thesis is part of the collection entitled: UNT Theses and Dissertations and was provided to Digital Library by the UNT Libraries.
Extracted Text
The following text was automatically extracted from the image on this page using optical character recognition software:
m
manna*. fli® original solution and fcb© two m*biag« «•« #©«-
tela®# to make total of itwn si* «f solution.
fanral.# of chloroform «*** added ta tha jsSjetwa of
inai solation of buffer isA ataroid and the few© waskingsj* fha
tuba* *«« ©appad and giwn ®& sfaak## bj brad p.iaead In feist
aba tear foa? $ aimttoa at ISO ppa ted than given SO nova abates
band* Tba tuba* war® than oantMfttga# foa* 5 isin&fcaa *1
lOOO <vpn* ■ fhe ®h%m®£®m (batten) Imm wifta a
-aail to%lb and pal- in a' claan tuba# . &ffc«r tbe first
«t efci«. of eti Iodoform w « raaoirad, e *1* ©f fresh ©hloro-
form were added to tb ' origin® 1, aolmiiran .ami na-abiagai -fetg a
second *xtv«etie«u< Again the efeler&fevat ls.3w.waa rewnred
after thorough adbiiag moA «d#ai. fee the £!*«%' - Tbe
tube* eonfcfiining efalorofona we* @ toft tightly: ©appad «• anob
M poaaibla I© prevent «v*pe* tiw of chloroform wbi#ii might
elte* the eeneeatretien of steroid, the tube* containing feto©
ohl©r©foi«st amtraotiona were taveyfee* itee®. tlaee to isssare
tfsjal, dlfttvlbaticni of the steroid •
'' tee si* of ®itlm®tmm aolutian wee veueved and placed in -
a tuba tm evaporation* 4 dtipiloat® simple of ehlovtifens waa
placed in toolbar trnbe to be aaed as # bettolegeu* blank# fhe
twe aata of uaeepped tobee, Sat I and Safe II, eaofa oontaiatag
1 *1# of *hl4eof«Mr ttolukien* wey* pleeed in a d®*i#fst#* whloh
mmn in a «#l®f bath at 6® degseee Centigrade*' She 6e«ls*ftt«*,
waa oonnactad to. the mmma pimp tteromgto a dry ice toafth de-
signed to ®«#e #e tha eblevefora and thus present reeetien
Upcoming Pages
Here’s what’s next.
Search Inside
This thesis can be searched. Note: Results may vary based on the legibility of text within the document.
Tools / Downloads
Get a copy of this page or view the extracted text.
Citing and Sharing
Basic information for referencing this web page. We also provide extended guidance on usage rights, references, copying or embedding.
Reference the current page of this Thesis.
Pryor, Nan Webb. Metabolism of Hydrocortisone by X-Irradiated Rat Liver Tissue as Determined by the Porter-Silber Chromagen Method, thesis, August 1959; Denton, Texas. (digital.library.unt.edu/ark:/67531/metadc108062/m1/15/: accessed February 18, 2019), University of North Texas Libraries, Digital Library, digital.library.unt.edu; .