Quantitative mammalian cell genetic toxicology: study of the cytotoxicity and mutagenicity of 70 individual environmental agents related to energy technologies and 3 subfractions of a crude synthetic oil in the CHO/HGPRT system. [Hamsters] Metadata

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Title

  • Main Title Quantitative mammalian cell genetic toxicology: study of the cytotoxicity and mutagenicity of 70 individual environmental agents related to energy technologies and 3 subfractions of a crude synthetic oil in the CHO/HGPRT system. [Hamsters]

Creator

  • Author: Hsie, A. W.
    Creator Type: Personal
  • Author: Neill, J. P.
    Creator Type: Personal
  • Author: San Sebastian, J. R.
    Creator Type: Personal

Publisher

  • Name: Oak Ridge National Laboratory
    Place of Publication: Tennessee
  • Name: Tennessee Univ., Oak Ridge (USA). School of Biomedical Sciences
    Place of Publication: United States

Date

  • Creation: 1978-01-01

Language

  • English

Description

  • Content Description: Conditions necessary for quantifying mutation-induction to 6-thioguanine resistance, which selects for >98% mutants deficient in the activity of hypoxanthine-guanine phosphoribosyl transferase (HGPRT) in a near-diploid Chinese hamster ovary (CHO) cell line, referred to as CHO/HGPRT system, have been defined. Employing this mutation assay, we have determined the mutagenicity of diversified agents including 11 direct-acting alkylating agents, 16 nitrosamines, 10 heterocyclic nitrogen mustards, 15 metallic compounds, 5 quinolines, 5 aromatic amines, 27 polycyclic hydrocarbons, 13 miscellaneous chemicals, 7 ionizing and non-ionizing physical agents. The direct-acting carcinogen N-methyl-N'-nitro-N-nitrosoguanidine is mutagenic while its noncarcinogenic analogue N-methyl-N'-nitro-N-nitroguanidine is not. Coupled with the rat liver S/sub 9/-activation system, procarcinogens such as nitrosopyrrolidine, benzo(a)pyrene, and 2-acetylaminofluorene are mutagenic while their analogues 2,5-dimethylnitrosopyrrolidine, pyrene and fluorene are not. The assay appears to be applicable for monitoring the genetic toxicity of crude organic mixtures in addition to diverse individual chemical and physical agents. The quantitative nature of the assay enables a study of EMS exposure dose: the mutagenic potential of EMS can be described as 310 x 10/sup -6/ mutants (cell mg ml/sup -1/ h)./sup -1/ It is also feasible to expand the CHO/HGPRT system for quantifying cytotoxicity and mutagenicity to determination of chromosomal aberrations and sister chromatid exchanges in cells treated under identical conditions which allows a simultaneous study of these four distinctive biological effects.

Subject

  • Keyword: Body
  • Keyword: Ovaries
  • Keyword: Amines
  • Keyword: Mammals
  • Keyword: Gonads
  • Keyword: Chromosomal Aberrations
  • Keyword: Nitrogen Mustard
  • Keyword: Drugs
  • Keyword: Biology
  • Keyword: Aldehydes
  • Keyword: Formaldehyde
  • Keyword: Organic Chlorine Compounds
  • Keyword: Genetic Effects
  • Keyword: Cytology
  • STI Subject Categories: 63 Radiation, Thermal, And Other Environ. Pollutant Effects On Living Orgs. And Biol. Mat.
  • Keyword: Pyridines
  • Keyword: Condensed Aromatics
  • Keyword: Mutagenesis
  • Keyword: Ems
  • Keyword: Animal Cells
  • Keyword: Antimitotic Drugs
  • Keyword: Female Genitals
  • Keyword: Sulfonic Acid Esters
  • Keyword: Toxicity
  • Keyword: Mutagens
  • Keyword: Organic Sulfur Compounds
  • Keyword: Alkylating Agents
  • Keyword: Hydrocarbons
  • Keyword: Fluorene
  • Keyword: Animals
  • Keyword: Carcinogens
  • Keyword: Organic Nitrogen Compounds
  • Keyword: Aromatics
  • STI Subject Categories: 59 Basic Biological Sciences
  • Keyword: Mutations
  • Keyword: Azines
  • Keyword: Heterocyclic Compounds
  • Keyword: Organic Compounds
  • Keyword: Organs
  • STI Subject Categories: 560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
  • Keyword: Quinolines
  • Keyword: Genetics
  • Keyword: Hamsters
  • Keyword: Organic Halogen Compounds
  • Keyword: Biological Effects
  • Keyword: Bioassay
  • Keyword: Vertebrates 550400* -- Genetics
  • Keyword: Rodents
  • Keyword: Esters

Collection

  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI

Institution

  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Report

Format

  • Text

Identifier

  • Report No.: CONF-780227-5
  • Grant Number: W-7405-ENG-26
  • Office of Scientific & Technical Information Report Number: 5114391
  • Archival Resource Key: ark:/67531/metadc1061464

Note

  • Display Note: Dep. NTIS, PC A03/MF A01.