Removal of uv-induced pyrimidine dimers from the replicated and unreplicated DNA of human fibroblasts Page: 3 of 4
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THE PRECENT OF DNA SYNTHESISED IN UV IRRADIATED HUMAN FIBRO-
Time after irradiation (hours
UV dose (J m-2) 12 hrs 24 hrs
0 100 100
5 56 10 85 13
10 25 6 50 * 6
Incidences of Pyrimidine Dimers and Repair Replication
in Replicated and Unreplicated DNA: Table 2 presents the
numbers of dimers left in the DNA replicated or remaining
unreplicated at 12 and 24 hour after irradiation. Slightly
fewer dimers were frequently observed in replicated ::s'
opposed to unreplicated strands but differences never exceed-
THE INCIDENCE OF PYRIMIDINE DINERS AND EXTENT OF REPAIR
REPLICATION IN DNA REPLICATED (HiL), OR REMAINING UNREPLICATED
(LL) AFTER UV.a
UV Dose (J-m-2) Time (hrs) 'per 108 daltons 3H/32p
HL LL HL LL
5 12 8 7 1.4 1.5
10 12 12 14 2.0 1.8
5 24 4 5 1.8 2.1
10 24 9 10 3.0 3.0
aTreatments were as indicated in the text and time
refers to the length of post UV incubation in BrdUrd contain-
ing medium. The number of dimers immediately after UV is
3.0/108/J-M-2. 3H/32P denotes the degree of excision
resynthesis associated with light DNA strands.
Hence, regardless of dose or time after UV, dimers are found
in both the replicated and unreplicated DNA. Following
10 J-m2 the DNA replicated during 12 hours after irradiation
was incubated with or without UV endonuclease. The heavy
daughter strand was larger than the interdimer distance
observed in the replicated light strand.
Table 2 also gives the amount of repair synthesis in the
replicated and unreplicated portions of the DNA that received
UV. The data indicate that after a given dose and post
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Waters, R. Removal of uv-induced pyrimidine dimers from the replicated and unreplicated DNA of human fibroblasts, article, January 1, 1978; Tennessee. (digital.library.unt.edu/ark:/67531/metadc1059269/m1/3/: accessed October 16, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.