The hemicellulases from the ethanologenic thermophile: Themoanaerobacter ethanolius

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Previously, we had only obtained extremely low xylanase activity in cultures of {ital Thermoanaerobacter ethanolicus} strain JW200, despite demonstrated xylan hydrolysis. We were not able to increase the enzyme activity or concentrate it in solution. Therefore, we decided to isolate new strains of thermophilic anaerobes with higher xylanase activity as a future source for cloning xylanases into {ital T. ethanolicus}. We now have organisms exhibiting 100-fold higher xylanase activity than JW200, but still cannot isolate or concentrate the enzyme activity except at very low yields. We have concentrated and partially purified a xylanase from strain N.D. using preparative matrix-free isoelectric ... continued below

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Pages: (6 p)

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Wiegel, J. May 1, 1991.

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Description

Previously, we had only obtained extremely low xylanase activity in cultures of {ital Thermoanaerobacter ethanolicus} strain JW200, despite demonstrated xylan hydrolysis. We were not able to increase the enzyme activity or concentrate it in solution. Therefore, we decided to isolate new strains of thermophilic anaerobes with higher xylanase activity as a future source for cloning xylanases into {ital T. ethanolicus}. We now have organisms exhibiting 100-fold higher xylanase activity than JW200, but still cannot isolate or concentrate the enzyme activity except at very low yields. We have concentrated and partially purified a xylanase from strain N.D. using preparative matrix-free isoelectric focusing. We have also purified to homogeneity and partially characterized a xylosidase from {ital T. ethanolicus}. We have detected and measured arabinosidase and acetyl esterase activity in {ital T.ethanolicus}, {ital Clostridium thermohydrosulfuricum} and strain N.D. 7 refs., 2 tabs. (MHB)

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Pages: (6 p)

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  • Other: DE92001859
  • Report No.: DOE/ER/14059-1
  • Grant Number: FG09-89ER14059
  • DOI: 10.2172/5012379 | External Link
  • Office of Scientific & Technical Information Report Number: 5012379
  • Archival Resource Key: ark:/67531/metadc1059136

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  • May 1, 1991

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  • Jan. 22, 2018, 7:23 a.m.

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  • Jan. 26, 2018, 5:48 p.m.

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Wiegel, J. The hemicellulases from the ethanologenic thermophile: Themoanaerobacter ethanolius, report, May 1, 1991; United States. (digital.library.unt.edu/ark:/67531/metadc1059136/: accessed April 27, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.