Cell-mediated mutagenesis by chemical carcinogens

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The cell-mediated mutation system, with the proper choice of metabolizing cells, can be used to detect the mutagenic activities of different classes of chemical carcinogens. When fibroblastic cells were used as the metabolizing cells, a correlation between the in vivo carcinogenic activity and the in vitro mutagenic activity of 11 aromatic polycyclic hydrocarbons was observed. When primary liver cells were used as the metabolizing cells, three known liver carcinogens were demonstrated to be mutagenic by the cell-mediated assay, while two non-carcinogenic analogues were not mutagenic. These results from the cell-mediated system suggest that the reactive intermediates of the carcinogens are ... continued below

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Pages: 14

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Huberman, E. & Langenbach, R. January 1, 1978.

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Description

The cell-mediated mutation system, with the proper choice of metabolizing cells, can be used to detect the mutagenic activities of different classes of chemical carcinogens. When fibroblastic cells were used as the metabolizing cells, a correlation between the in vivo carcinogenic activity and the in vitro mutagenic activity of 11 aromatic polycyclic hydrocarbons was observed. When primary liver cells were used as the metabolizing cells, three known liver carcinogens were demonstrated to be mutagenic by the cell-mediated assay, while two non-carcinogenic analogues were not mutagenic. These results from the cell-mediated system suggest that the reactive intermediates of the carcinogens are stable enough to be transferred from the metabolizing cells to the V79 cells. The cell-mediated mutagenesis system is a simple in vitro assay which may simulate the in vivo situation. It was concluded that this approach could be extended to the co-cultivation of cells from other organs or tissues with mutable mammalian cells.

Physical Description

Pages: 14

Notes

Dep. NTIS, PC A02/MF A01.

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  • Symposium on application of short-term bioassays in the fractionation and analysis of complex environmental mixtures, Williamsburg, VA, USA, 21 Feb 1978

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  • Report No.: CONF-780227-4
  • Grant Number: W-7405-ENG-26
  • Office of Scientific & Technical Information Report Number: 5087274
  • Archival Resource Key: ark:/67531/metadc1052816

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • January 1, 1978

Added to The UNT Digital Library

  • Jan. 22, 2018, 7:23 a.m.

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  • Feb. 1, 2018, 6:04 p.m.

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Huberman, E. & Langenbach, R. Cell-mediated mutagenesis by chemical carcinogens, article, January 1, 1978; Tennessee. (digital.library.unt.edu/ark:/67531/metadc1052816/: accessed October 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.