Applications of carbon-13 and sodium-23 NMR in the study of plants, animal, and human cells

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Carbon-13 and sodium-23 NMR have been applied to the study of a variety of plant, animal and human cell types. Sodium NMR, in combination with dysprosium shift reagents, has been used to monitor sodium transport kinetics in salt-adapted, and non-adapted cells of P. milliaceum and whole D. spicata plants. The sodium content of human erythrocytes and leukemic macrophages was measured. Carbon-13 NMR was used to determine the structure and metabolism of rat epididymal fat pad adipocytes in real time. Insulin and isoproterenol-stimulated triacylglycerol turnover could be monitored in fat cell suspensions. (1-/sup 13/C) glucose was used as a substrate to ... continued below

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Pages: 26

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Sillerud, L.O.; Heyser, J.W.; Han, C.H. & Bitensky, M.W. January 1, 1984.

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Carbon-13 and sodium-23 NMR have been applied to the study of a variety of plant, animal and human cell types. Sodium NMR, in combination with dysprosium shift reagents, has been used to monitor sodium transport kinetics in salt-adapted, and non-adapted cells of P. milliaceum and whole D. spicata plants. The sodium content of human erythrocytes and leukemic macrophages was measured. Carbon-13 NMR was used to determine the structure and metabolism of rat epididymal fat pad adipocytes in real time. Insulin and isoproterenol-stimulated triacylglycerol turnover could be monitored in fat cell suspensions. (1-/sup 13/C) glucose was used as a substrate to demonstrate futile metabolic cycling from glucose to glycerol during lypolysis. Cell wall polysaccharide synthesis was followed in suspensions of P. milliaceum cells using (1-/sup 13/C) glucose as a precursor. These results illustrate the wide range of living systems which are amenable to study with NMR. 14 refs., 21 figs.

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Pages: 26

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NTIS, PC A03/MF A01.

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  • NATO advanced study institute, Palermo, Sicily, 1 Aug 1984

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  • Other: DE86000800
  • Report No.: LA-UR-85-3447
  • Report No.: CONF-8408176-1
  • Grant Number: W-7405-ENG-36
  • Office of Scientific & Technical Information Report Number: 5043534
  • Archival Resource Key: ark:/67531/metadc1052401

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  • January 1, 1984

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  • Jan. 22, 2018, 7:23 a.m.

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  • Feb. 1, 2018, 6:59 p.m.

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Sillerud, L.O.; Heyser, J.W.; Han, C.H. & Bitensky, M.W. Applications of carbon-13 and sodium-23 NMR in the study of plants, animal, and human cells, article, January 1, 1984; New Mexico. (digital.library.unt.edu/ark:/67531/metadc1052401/: accessed November 13, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.