Characterization of UGT716A1 as a Multi-substrate UDP:Flavonoid Glucosyltransferase Gene in Ginkgo biloba

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This article discusses the generation of a transcriptomic dataset of G. biloba leaf tissue by high-throughput RNA sequencing to better understand flavonoid glucosylation in G. biloba.

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15 p.

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Su, Xiaojia; Shen, Guoan; Di, Shaokang; Dixon, R. A. & Pang, Yongzhen December 7, 2017.

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  • Su, Xiaojia Chinese Academy of Sciences; Chinese Academy of Agricultural Sciences
  • Shen, Guoan Chinese Academy of Sciences
  • Di, Shaokang Chinese Academy of Sciences
  • Dixon, R. A. University of North Texas; Beijing Forestry University
  • Pang, Yongzhen Chinese Academy of Sciences; Chinese Academy of Agricultural Sciences

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This article discusses the generation of a transcriptomic dataset of G. biloba leaf tissue by high-throughput RNA sequencing to better understand flavonoid glucosylation in G. biloba.

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15 p.

Notes

Abstract: Ginkgo biloba L., a “living fossil” and medicinal plant, is a well-known rich source
of bioactive flavonoids. The molecular mechanism underlying the biosynthesis of
flavonoid glucosides, the predominant flavonoids in G. biloba, remains unclear. To
better understand flavonoid glucosylation in G. biloba, we generated a transcriptomic
dataset of G. biloba leaf tissue by high-throughput RNA sequencing. We identified 25
putative UDP-glycosyltransferase (UGT) unigenes that are potentially involved in the
flavonoid glycosylation. Among them, we successfully isolated and expressed eight
UGT genes in Escherichia coli, and found that recombinant UGT716A1 protein was
active toward broad range of flavonoid/phenylpropanoid substrates. In particular, we
discovered the first recombinant UGT protein, UGT716A1 from G. biloba, possessing
unique activity toward flavanol gallates that have been extensively documented to have
significant bioactivity relating to human health. UGT716A1 expression level paralleled
the flavonoid distribution pattern in G. biloba. Ectopic over-expression of UGT716A1
in Arabidopsis thaliana led to increased accumulation of several flavonol glucosides.
Identification and comparison of the in vitro enzymatic activity of UGT716A1 homologs
revealed a UGT from the primitive land species Physcomitrella patens also showed
broader substrate spectrum than those from higher plants A. thaliana, Vitis vinifera, and
Medicago truncatula. The characterization of UGT716A1 from G. biloba bridges a gap
in the evolutionary history of UGTs in gymnosperms. We also discuss the implication
of UGT716A1 for biosynthesis, evolution, and bioengineering of diverse glucosylated
flavonoids.

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  • Frontiers in Plant Science, 2017. Lausanne, Switzerland: Frontiers Research Foundation

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  • Publication Title: Frontiers in Plant Science
  • Volume: 8
  • Pages: 1-15
  • Peer Reviewed: Yes

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UNT Scholarly Works

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Submitted Date

  • September 29, 2017

Accepted Date

  • November 22, 2017

Creation Date

  • December 7, 2017

Added to The UNT Digital Library

  • Dec. 14, 2017, 11:26 a.m.

Description Last Updated

  • Feb. 21, 2019, 8:15 p.m.

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Su, Xiaojia; Shen, Guoan; Di, Shaokang; Dixon, R. A. & Pang, Yongzhen. Characterization of UGT716A1 as a Multi-substrate UDP:Flavonoid Glucosyltransferase Gene in Ginkgo biloba, article, December 7, 2017; Lausanne, Switzerland. (https://digital.library.unt.edu/ark:/67531/metadc1049702/: accessed June 25, 2019), University of North Texas Libraries, Digital Library, https://digital.library.unt.edu; crediting UNT College of Arts and Sciences.