Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris

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D. vulgaris Hildenborough has 72 response regulators. The Desulfovibrio are sulfate reducing bacteria that are important in the sulfur and carbon cycles in anoxic habitats. Its large number of two componenent systems are probably critical to its ability to sense and respond to its environment. Our goal is to map these RRs to the genes they regulate using a DNA-affinity-purification-chip (DAP-chip) protocol. First target determined usuing EMSA. A positive target was determined for as many RRs as possible using EMSA. Targets were selected based on gene proximity, regulon predictions and/or predicted sigma54 dependent promoters. qPCR was used to ensure that ... continued below

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Rajeev, Lara; Luning, Eric; Dehal, Paramvir; Joachimiak, Marcin & Mukhopadhyay, Aindrila May 17, 2010.

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D. vulgaris Hildenborough has 72 response regulators. The Desulfovibrio are sulfate reducing bacteria that are important in the sulfur and carbon cycles in anoxic habitats. Its large number of two componenent systems are probably critical to its ability to sense and respond to its environment. Our goal is to map these RRs to the genes they regulate using a DNA-affinity-purification-chip (DAP-chip) protocol. First target determined usuing EMSA. A positive target was determined for as many RRs as possible using EMSA. Targets were selected based on gene proximity, regulon predictions and/or predicted sigma54 dependent promoters. qPCR was used to ensure that the target was enriched from sheared genomic DNA before proceeding to the DAP-chip.

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  • 110th General Meeting of the American Society for Microbiology, San Diego, CA, May 2010

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  • Report No.: LBNL-3687E-Poster
  • Grant Number: DE-AC02-05CH11231
  • DOI: 10.2172/985735 | External Link
  • Office of Scientific & Technical Information Report Number: 985735
  • Archival Resource Key: ark:/67531/metadc1013270

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  • May 17, 2010

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  • Oct. 14, 2017, 8:36 a.m.

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  • Oct. 18, 2017, 10:32 a.m.

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Rajeev, Lara; Luning, Eric; Dehal, Paramvir; Joachimiak, Marcin & Mukhopadhyay, Aindrila. Mapping the Two-component Regulatory Networks in Desulfovibrio vulgaris, report, May 17, 2010; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc1013270/: accessed October 17, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.