Self-organization of engineered epithelial tubules by differential cellular motility

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Patterning of developing tissues arises from a number of mechanisms, including cell shape change, cell proliferation, and cell sorting from differential cohesion or tension. Here, we reveal that differences in cell motility can also lead to cell sorting within tissues. Using mosaic engineered mammary epithelial tubules, we found that cells sorted depending on their expression level of the membrane-anchored collagenase matrix metalloproteinase (MMP)-14. These rearrangements were independent of the catalytic activity of MMP14 but absolutely required the hemopexin domain. We describe a signaling cascade downstream of MMP14 through Rho kinase that allows cells to sort within the model tissues. Cell ... continued below

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Mori, Hidetoshi; Gjorevski, Nikolce; Inman, Jamie L; Bissell, Mina J & Nelson, Celeste M February 4, 2009.

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Patterning of developing tissues arises from a number of mechanisms, including cell shape change, cell proliferation, and cell sorting from differential cohesion or tension. Here, we reveal that differences in cell motility can also lead to cell sorting within tissues. Using mosaic engineered mammary epithelial tubules, we found that cells sorted depending on their expression level of the membrane-anchored collagenase matrix metalloproteinase (MMP)-14. These rearrangements were independent of the catalytic activity of MMP14 but absolutely required the hemopexin domain. We describe a signaling cascade downstream of MMP14 through Rho kinase that allows cells to sort within the model tissues. Cell speed and persistence time were enhanced by MMP14 expression, but only the latter motility parameter was required for sorting. These results indicate that differential directional persistence can give rise to patterns within model developing tissues.

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  • Journal Name: Proceedings of the National Academy of Sciences; Journal Volume: 106; Journal Issue: 35; Related Information: Journal Publication Date: September 1, 2009

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  • Report No.: LBNL-3878E
  • Grant Number: DE-AC02-05CH11231
  • Grant Number: CA057621, CA064786, CA112970, CA126552, GM083997, and CA128660
  • DOI: 10.1073/pnas.0901269106 | External Link
  • Office of Scientific & Technical Information Report Number: 986920
  • Archival Resource Key: ark:/67531/metadc1012243

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • February 4, 2009

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  • Oct. 14, 2017, 8:36 a.m.

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  • Oct. 18, 2017, 10:11 a.m.

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Mori, Hidetoshi; Gjorevski, Nikolce; Inman, Jamie L; Bissell, Mina J & Nelson, Celeste M. Self-organization of engineered epithelial tubules by differential cellular motility, article, February 4, 2009; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc1012243/: accessed December 17, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.