Search Results

Studies on Tissue Factor Pathway Inhibitor in Zebrafish

Description: Tissue Factor Pathway Inhibitor (TFPI) is an anticoagulant protein containing three Kunitz domains, K1, K2 and K3. K1 inhibits Factor VIIa, K2 inhibits Factor Xa, and K3 enhances the Factor Xa inhibition by its interaction with Protein S. Since zebrafish is an excellent genetic model, we hypothesized that TFPI regulation could be studied using this model. As a first step, we confirmed the presence of tfpia in zebrafish. Subsequently, we performed knockdown of tfpia, and knockout of tfpia in K3 … more
Date: August 2021
Creator: Raman, Revathi
Partner: UNT Libraries
open access

Cardiovascular Fetal Programming in Quail (Colinus virginianus), An Avian Comparative Model

Description: The consequences of early embryonic insults and how they affect subsequent life reflects the emerging concept of "fetal programming". The aim of this project is to study the effects of embryonic insults as they subsequently manifest themselves in adults, with emphasis on the heart and vasculature. My experiments establish that fetal programming operates on the bobwhite quail inducing similar changes as those observed in mammalians and other birds. The quail's fast development provides reliable … more
Date: December 2016
Creator: Flores Santin, Josele R.
Partner: UNT Libraries
open access

Effect of pH on the Persistence and Toxicity of Cyfluthrin to Chironomus Tentans

Description: The effect of pH upon the aquatic toxicity of cyfluthrin was determined in 48 h static acute toxicity tests using 2nd instar Chironomus tentans larvae. Tests were conducted in both dechlorinated tap water and lake water of pH 8.0, 7.2, and 6.6. After 48 h, immobilized and dead larvae were removed and replaced with 2nd instar larvae to assess the persistence of toxicity. Midges were cultured in water adjusted to the pH values used in testing. Toxicity if cyfluthrin varied inversely with pH. An i… more
Date: May 1992
Creator: Hambleton, Faith Ann (Faith Ann Elizabeth)
Partner: UNT Libraries
open access

Advanced Techniques in Microbial and Molecular Biology: Laboratory Procedures for a Graduate Level Course

Description: Advanced laboratory techniques for Microbial and Molecular Biology at the graduate level are presented in this thesis. The procedures for the laboratory experiments are set forth in detail. This laboratory is conducted as two parts, each by a different professor. Part 1 covers the experiments conducted by Dr G.A.O. Donovan. These experiments include an introduction, staining procedures, biochemical reactions, mutagenesis experiment, essays,. preparation and analysis of plasmid DNA and various o… more
Date: August 1998
Creator: Kumar, Shalini
Partner: UNT Libraries
open access

A Water Quality Study of Lake Texoma

Description: A series of four stations for sampling the waters of the Red Rivers and Lake Texoma was established. Water samples from top, middle, and bottom levels of these four stations were taken on monthly intervals during periods of homothermousity and on two-week intervals during periods of thermal stratification. These samples were analysed for the cations sodium, potassium, calcium, and magnesium, and for the anions normal carbonate, bicarbonate, sulfate, chloride, nitrate, water hardness (expressed … more
Date: June 1959
Creator: Goodman, John Willis
Partner: UNT Libraries
open access

Toxicological Evaluation for the Ocular Administration of Tolrestat: An Aldose Reductase Inhibitor for the Treatment of Diabetes

Description: Aldose reductase inhibitors (ARI's) have been shown to attenuate or prevent several complications of diabetes in animals. Tolrestat is a potent and unique ARI from Ayerst Laboratories, New York, NY. The efficacy and toxicology of tolrestat via topical ocular administration was examined. Tolrestat effectively enhanced corneal reepithelialization and was efficacious in the prevention of cataracts in the streptozotocin-diabetic rat. Ocular tissues were examined by slit lamp biomicroscopy, scanning… more
Date: December 1987
Creator: Carney, Gerald R.
Partner: UNT Libraries
open access

Effects of Nutrient Media on Growth and Morphology of Azotobacter Vinelandii

Description: The work described in this thesis was undertaken to study the reasons why Azotobacter vinelandii ATCC 12837 after incubation in Burk's nitrogen-free liquid will not form as many colonies when plated on Difco Tryptic Soy Agar as when planted on Burk's nitrogen-free agar. The difference in growth of A. vinelandii on the two agars was established by performing viable cell-plate counts. The difference in growth was most apparent at 24-hours incubation of the Burk's liquid-media cultures. Phase cont… more
Date: August 1974
Creator: Butsch, Robert W.
Partner: UNT Libraries
open access

Assessment of Radio-Tagged Grass Carp (Ctenopharnygodon idella) Dispersion, Vegetation, and Temperature Preferences in North Lake Reservoir

Description: Twenty-nine (Group One, June 8,1995) grass carp (Ctenopharyngodon idella) and five (Group Two, April 18, 1996) grass carp were radio-tagged to monitor movement patterns and habitat preferences on North Lake, a 335 hectare multi-use reservoir located in Irving, Texas. Overall fish mean Average Daily Movement (ADM) rates were 49.2 meters/day (during Half One, 6/8/95-11/30/95) and 5.3 meters/day (during Half Two, 12/14/95-6/6/96). Aquatic macrophtye distribution data were obtained. Radio-tagged gr… more
Date: August 1996
Creator: Lacewell, Jason (Jason Lawrence)
Partner: UNT Libraries
open access

A Comparison of Thermogenesis by Selected Substrates on Hypothermic Rat Liver

Description: The thermogenic effects in hypothermia of four substrates--alanine, glycine, ethano, and pyruvate - were studied in seventeen experiments. Albino rats were decapitated, and their livers were removed. The livers were homogenized with phosphate buffer at -5° C. After equilibration in a refrigerated Warburg apparatus at 20° C, the substrates were added and tissue respiration was recorded over three hours. Heat production was calculated from O2 uptake and CO2 production. Results showed that alanine… more
Date: August 1979
Creator: Long, James T.
Partner: UNT Libraries
open access

Spatially Explicit Modeling of West Nile Virus Risk Using Environmental Data

Description: West Nile virus (WNV) is an emerging infectious disease that has widespread implications for public health practitioners across the world. Within a few years of its arrival in the United States the virus had spread across the North American continent. This research focuses on the development of a spatially explicit GIS-based predictive epidemiological model based on suitable environmental factors. We examined eleven commonly mapped environmental factors using both ordinary least squares regress… more
Date: December 2015
Creator: Kala, Abhishek K.
Partner: UNT Libraries
open access

Impact of a Genetically Engineered Probiotic Therapy and IGF-1 Genomics in the PAHenu2 Mouse Model of PKU

Description: Absence of functional phenylalanine hydroxylase results in phenylketonuria (PKU). Viable treatments remain few, expensive and secondary conditions such as osteopenia occur in most PKU patients. Objective 1: Given the recently described roles of gut microbes to aid host digestion, an orally administered genetically engineered probiotic as the delivery vehicle for enzyme replacement therapy was created. The engineered probiotic, pHENOMMenal, produced phenylalanine ammonia lyase with significant… more
Date: December 2015
Creator: Durrer, Katherine Elaine
Partner: UNT Libraries
open access

Genetic Analysis of Medicago truncatula Plants with a Defective MtIRE Gene

Description: Leguminous plants are able to fix nitrogen by establishing a symbiotic relationship with soil dwelling bacteria, called rhizobia. The model plant Medicago truncatula forms a partnership with Sinorhizobium meliloti whereby the plant gains bioavailable nitrogen and in exchange the bacteria gains carbohydrates. This process occurs within nodules, which are structures produced on the roots of the plants within which nitrogen is fixed. M. truncatula incomplete root elongation (MtIRE) was localized t… more
Date: August 2015
Creator: Alexis, Naudin
Partner: UNT Libraries
open access

Forward Genetic Characterization of Medicago truncatula Tnt1 Insertion Mutants Defective in Nodule Development and Symbiotic Nitrogen Fixation

Description: Legumes are unique plants because they form special structures “nodules”, via symbiotic relationships with rhizobial bacteria present in the soil. Once rhizobia mature inside nodules, they fix atmospheric nitrogen providing a source of bioavailable nitrogen to the plant. To discover novel genetic components involved in the legume-rhizobia symbiosis by using forward genetic screening, we have isolated Medicago truncatula Tnt1 insertion mutants in the R108 ecotype, which are defective in nodule d… more
Date: May 2015
Creator: Kadel, Khem L.
Partner: UNT Libraries
open access

A Study of Some Congenital Anomalies of the Teeth

Description: Anomalies of the teeth include a variety of expression such as: anodontia, hyperdontia, peg teeth. opalescent dentine, mottled enamel, and other imperfections of the teeth. This paper is concerned primarily with anodontia in human subjects, although other abnormalities of interest are mentioned. Insufficient datum is the principal difficulty encountered in determining the mechanism and means of transmission in human genetics. Small families, slow breeding, and inability to subject humans to exp… more
Date: August 1955
Creator: Jones, Alvin Watt
Partner: UNT Libraries
open access

Relationship of Certain Fungi to Azotobacter in Nitrogen-Free Media

Description: Azotobacter and various fungi were grown together in nitrogen-free media. Maximal fungal growth in the medium used was possible only at the expense of Azotobacter cells and growth was always accompanied by acid production. When the medium reached a pH of 2, the bacterial cells were aggregated on fungal hyphae and the culture fluid appeared to be free of Azotobacter. Aspergillus niger grew well at the expense of viable bacteria and other fungi grew well on heat-killed cells of A. vinelandii. Mem… more
Date: August 1976
Creator: Ray, Manfred G.
Partner: UNT Libraries
open access

A Multiphasic Study of the Interaction Between the Branchiobdellid Cambarincola Vitrea and its Crayfish Host Procambarus Simulans

Description: The host-parasite interface between the branchiobdellid Cambarincola vitrea Ellis and its crayfish host is investigated along ecological, ultrastructural, serological, and endocrinological lines of evidence. Monthly analysis of a natural branchiobdellid from a vernal habitat demonstrates the annelid to be particularly sensitive to changes involving both the physical habitat as well as the host crayfish.
Date: August 1973
Creator: Koepp, Stephen John
Partner: UNT Libraries
open access

Thyroid Ultrastructural Changes Induced by Hypothermia

Description: Investigations have shown that the hypothalamus and pituitary respond to decreases in body temperature by stimulating the thyroid gland to release T3 and T4 hormones. This study was designed to investigate ultrastructural changes of the thyroid gland induced by hypothermia. The ultrastructural changes produces by stimulation by Thyroid Stimulating Hormone were also examined as an adjunct to the hypothermic stimulation of the gland. There was a significant increase in microvilli on the luminal b… more
Date: August 1979
Creator: Kent, James Simpson
Partner: UNT Libraries
open access

An Evaluative Study of the Grasslands of the R.J. McMurry Ranch, Denton County, Texas

Description: It is the purpose of this problem to classify the four major pastures of the McMurry ranch using the discussed classification system. The definite measurable qualities characterizing each condition of the system are used extensively in this study. The problem is concerned also with the observation of results of misuse, the present practices that could result in further depletion of portions of the McMurry ranch, and procedures being employed at the present time aimed toward the restoration of t… more
Date: June 1951
Creator: Nolen, Bette Rudd
Partner: UNT Libraries
open access

A Quantitative and Qualitative Bacterial Analysis of the Sewage Disposal System of Perryton, Texas

Description: This investigation had for its purpose the determination of first, the inefficiency of the Perryton, Texas, sewage disposal plant proper; and second the potential effectiveness of a lagoon now used to complete the sewage purification; and consequently, offset an apparent health hazard. The problem, as pursued, has consisted of first, the collection of sewage samples from the six stations listed in Table 1 over the period of time from September 23, 1949 to May 14, 1950; second, a quantitative an… more
Date: August 1950
Creator: Sullivan, Troy G.
Partner: UNT Libraries
open access

Development of Range Condition Classes for Coleman County, Texas

Description: This paper deals with a study of the rangelands in Coleman County just west south and west of the Western Cross Timbers of Texas. This problem consists of classifying certain rangelands in this area according to whether they are excellent, fair, good, or poor, the chief basis for which is their deterioration.
Date: June 1951
Creator: McMinn, Philip Morris
Partner: UNT Libraries
open access

Investigating the Ability of Pseudomonas aeruginosa pyrE Mutants to Grow and Produce Virulence Factors

Description: Pseudomonas aeruginosa are medically important bacteria that are notorious for causing nosocomial infections. To gain more knowledge into understanding how this organism operates, it was decided to explore the pyrimidine biosynthetic pathway. Pyrimidine synthesis, being one half of the DNA structure, makes it a very important pathway to the organism’s survivability. With previous studies being done on various genes in the pathway, pyrE has not been studied to the fullest extent. To study the fu… more
Date: December 2014
Creator: Niazy, Abdurahman
Partner: UNT Libraries
open access

The Impact of Developmental Stress on Cardiovascular Physiology of Two Archosaur Species: American Alligator (Alligator mississippiensis) and Domestic Chicken (Gallus gallus)

Description: Crocodilians and birds comprise sister taxa of archosaurs, the development of these vertebrates occurs within an egg case that leaves developing embryos susceptible to fluctuations in the nesting environment. Studies suggest that sub-optimal conditions alter morphological growth and cardiovascular physiology. Regulation of the cardiovascular system is immature in the subjects studied, and embryos may rely on humoral rather than neural control of the cardiovascular system. The primary focus of t… more
Date: December 2014
Creator: Tate, Kevin B.
Partner: UNT Libraries
open access

The Effects of Common Electrolytes on Growth and Development of Selected Species of Aquatic Actinomycetes

Description: The role that aquatic actinomycetes play, in the production of tastes and odors in water supplies has been investigated since 1948. The ability of these organisms to produce by-products in lakes and streams, which renders the water unpalatable, is of considerable public health importance. It is desirable that the waterworks industry has as much information as possible concerning the factors that contribute to the growth of these organisms. Since it appears that the aquatic actinomycetes may be … more
Date: August 1959
Creator: Sissom, Stanley L.
Partner: UNT Libraries
Back to Top of Screen