Analysis of the Expression Profiles of Two Isoforms of the Antifungal Protein Osmotin from Gossypium hirsutum

Analysis of the Expression Profiles of Two Isoforms of the Antifungal Protein Osmotin from Gossypium hirsutum

Date: May 2007
Creator: Spradling, Kimberly Diane
Description: The expression of two cotton osmotin genes was evaluated in terms of the mRNA and protein expression patterns in response to chemical inducers such as ethylene, hydrogen peroxide, and sodium chloride. Reverse transcriptase-polymerase chain reactions (RT-PCR) indicated that osmotin mRNAs are expressed constitutively in root tissues of cotton plants, and that they are rapidly induced in leaf and stem tissues upon ethylene treatment. Real time RT-PCR indicated that osmotin transcript levels were induced 2 to 4 h after treatment with ethephon. The osmotin mRNA levels appear to increase 12 h after treatment, decrease, and then increase again. The osmotin protein expression patterns were analyzed in Western blot analyses using an anti-osmotin antibody preparation. A 24-KDa protein band was detected from cotton plants treated with the inducers. The 24-KDa osmotin proteins were induced 4 h after treatment with ethephon, while down-regulated 96 h after treatment. Multiple osmotin isoforms were observed to be induced in cotton plants upon treatment with ethephon by two-dimensional gel electrophoresis. One goal of this dissertation research was to genetically engineer two cotton osmotin genes to routinely overproduce their antifungal proteins in transgenic Arabidopsis and cotton plants as a natural defense against fungal infections, using co-cultivation with Agrobacterium ...
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A Chorus of Trees

A Chorus of Trees

Access: Use of this item is restricted to the UNT Community.
Date: August 2010
Creator: Lyons, Renée Kathleen
Description: This two-part thesis includes a critical preface and a collection of my poems. Using three poems-Louise Glück's "Lullaby," Bob Hicok's "Poem for My Mother's Hysterectomy," and Nick Flynn's "Memento Mori"-the critical preface examines how, in poetry, the transformation of a body negotiates trauma and triggers a conceptual shift, the creation and revision of identity, and the release of the duende's inspirational force. The collection of poetry that follows seeks to transfigure the body as a way to explore the nuanced traumas of human experience.
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The Transformation of Materials and Representation of the Idea of the Baby Doll

The Transformation of Materials and Representation of the Idea of the Baby Doll

Date: August 2003
Creator: Wehring, Brad
Description: I want to find a balance within the juxtaposition of representational imagery, patterned fabric, stain and found objects, which effectively communicates the ideas of my work, yet still provides a visually interesting object/painting. How do my materials relate to the content and/or meaning of the work? How will focusing on a single subject affect the development and visual content of my painting? How will I choose representational images to use in relation to the aims of my subject? I was struck by the connections between the baby doll and the real baby. The baby doll became a representation of an idealized body. My interest in baby doll source materials evolved through several different stages, beginning with drawings of baby dolls, then actual doll parts, and finally to imagery of babies with genetic defects. Formally, the work was able to progress as the idea or content progressed.
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Constructing transformative experiences through problem posing in a high school English research project.

Constructing transformative experiences through problem posing in a high school English research project.

Date: May 2009
Creator: Revelle, Carol L.
Description: This dissertation chronicles my search to engage high school English students in inquiry as part of a formal research process. The perspective of critical literacy theory is used to describe the four phases of the problem posing process in shaping student research and action. Grounded in Freire's approach and consistent with Dewey and others who advocate inquiry, action and relevance, Wink's process is built into the instructional plan described in this study. Because of the real-life context of the classroom and the complex social phenomena being considered, a case study methodology was utilized in which multiple sources of data converged to develop the themes. Data sources included the work and artifacts of ten students in a tenth grade English class during the spring semester of 2008. The analysis focuses on the supports, the constraints and the impact of problem posing on the high school research assignment. The analysis, findings, and conclusions contribute to the literature in three areas: audience, reflection and grading.
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Forsythia

Forsythia

Date: December 2005
Creator: Pike, Brianna J.
Description: Forsythia is a collection of poetry that examines the transformative power of observation.
Contributing Partner: UNT Libraries