Zebrafish Von Willebrand Factor

Zebrafish Von Willebrand Factor

Date: August 2012
Creator: Carrillo, Maira M.
Description: In humans, von Willebrand factor (vWF) is a key component in hemostasis and acts as a 'cellular adhesive' by letting the circulating platelets bind to exposed subendothelium. It also acts as a carrier and stabilizer of factor VIII (FVIII). A dysfunction or reduction of vWF leads to von Willebrand disease (vWD), resulting in bleeding phenotype which affects 1% of the population. Currently there are a variety of animal models used for the study of vWF and vWD; however, they do not possess the advantages found in zebrafish. Therefore, we set out to establish zebrafish as a model for the investigation of vWF and vWD through the use of bioinformatics and various molecular techniques. Using bioinformatics we found that the vWF gene is located on chromosome 18, that the GPIb? protein sequence is conserved. Confirmation of vWF production was shown by means of immunostaining and by RT-PCR, in thrombocytes as well as in veins and arteries. Evidence of vWF involvement in hemostasis and thrombosis was shown using MO and VMO technology to produce a vWD like phenotype, resulting in an increase in TTO and TTA, as well as a reduction in FVIII when blood was tested using the kPTT assay, coinciding ...
Contributing Partner: UNT Libraries
Zebrafish Thrombocytes: Functions and Origins

Zebrafish Thrombocytes: Functions and Origins

Date: 2012
Creator: Khandekar, Gauri; Kim, Seongcheol & Jagadeeswaran, Pudur
Description: Article integrating the information available on zebrafish thrombocyte function and its formation.
Contributing Partner: UNT College of Arts and Sciences
G6f-Like Is an ITAM-Containing Collagen Receptor in Thrombocytes

G6f-Like Is an ITAM-Containing Collagen Receptor in Thrombocytes

Date: December 2012
Creator: Hughes, Craig E.; Radhakrishnan, Uvaraj P.; Lordkipanidzé, Marie; Egginton, S.; Dijkstra, Johannes M.; Jagadeeswaran, Pudur et al.
Description: Article discussing how G6f-like is an ITAM-containing collagen receptor in thrombocytes.
Contributing Partner: UNT College of Arts and Sciences