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Identification of Endogenous Substrates for ADP-Ribosylation in Rat Liver

Description: Bacterial toxins have been shown to modify animal cell proteins in vivo with ADPR. Animal cells also contain endogenous enzymes that can modify proteins. Indirect evidence for the existence in vivo of rat liver proteins modified by ADPR on arginine residues has been reported previously. Presented here is direct evidence for the existence of ADP-ribosylarginine in rat liver proteins. Proteins were subjected to exhaustive protease digestion and ADP-ribosyl amino acids were isolated by boronate ch… more
Date: May 1992
Creator: Loflin, Paul T. (Paul Tracey)
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries

Computational Modeling of Cancer-Related Mutations in DNA Repair Enzymes Using Molecular Dynamics and Quantum Mechanics/Molecular Mechanics

Description: This dissertation details the use of computational methods to understand the effect that cancer-related mutations have on proteins that complex with nucleic acids. Firstly, we perform molecular dynamics (MD) simulations of various mutations in DNA polymerase κ (pol κ). Through an experimental collaboration, we classify the mutations as more or less active than the wild type complex, depending upon the incoming nucleotide triphosphate. From these classifications we use quantum mechanics/molecula… more
Date: May 2022
Creator: Leddin, Emmett Michael
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

Protein Profiles of Azotobacter Vinelandii During the Encystment Process

Description: Azotobacter vinelandii 12837 was grown in Burk's glucose media and transferred onto Burk's n-butanol agar plates to allow for the formation of cysts. The patterns of the vegetative cell proteins were compared for each successive day of cyst formation, using the polyacrylamide gel isoelectric focusing technique. The findings revealed that, as the cysts developed to maturity, definite changes occurred in the protein constitution, indicative of the biochemical and physiological changes which cells… more
Date: August 1977
Creator: Butler, Mark A.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

The Effect of Toasting on the Protein Quality of White Bread and White Bread Supplemented with Soybean

Description: Supplemented and unsupplemented white breads were baked and toasted at three different toaster settings, light, medium, and dark. Organoleptically, products were highly accepted when toasted at the light and medium temperatures. Biological tests with rats resulted in a decline in the efficient utilization of the protein with toasted white bread diets, evidenced by poor weight gain, low liver weight, Serum protein, PER (protein efficiency ratio - weight gain/protein intake) and percent digestabi… more
Date: August 1979
Creator: Okoro, Chioma E.
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
open access

How Do Enzymes Wear Out? Effects of Posttranslational Modifications on Structure and Stability of Proteins; The Triosephosphate Isomerase Model

Description: Triosephosphate isomerase (EC 5.3.1.1., TPI) undergoes specific posttranslational modifications (deamidation and oxidation) which are believed to initiate protein turnover by destabilization of the dimer. The crystal structures, amino acid sequences, and aging related changes of TPI from various species have been independently characterized by several laboratories. TPI has thus become the prototype enzyme for examining the initial steps in protein turnover. The binding of substrate enhances the… more
Date: December 1991
Creator: Sun, An Qiang
Item Type: Refine your search to only Thesis or Dissertation
Partner: UNT Libraries
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