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Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport

Description: This article discusses functional characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter.
Date: September 2008
Creator: Srivastava, Avinash C.; Ganesan, Savita; Ismail, Ihab O. & Ayre, Brian G.
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Partner: UNT College of Arts and Sciences
open access

Temperature-sensitive Post-translational Regulation of Plant Omega-3 Fatty-acid Desaturases Is Mediated by the Endoplasmic Reticulum-associated Degradation Pathway

Description: Article expressing two closely related plant FAD3 genes in yeast cells and found that their enzymes produced significantly different amounts of omega-3 fatty acids and that these differences correlated to differences in rates of protein turnover. The findings indicate that Fad3 protein abundance is regulated by a combination of cis-acting degradation signals and the ubiquitin-proteasome pathway and that modulation of Fad3 protein amounts in response to temperature may represent one mechanism of… more
Date: July 9, 2010
Creator: O'Quin, Jami B.; Bourassa, Linda; Zhang, Daiyuan; Shockey, Jay M.; Gidda, Satinder K.; Fosnot, Spencer et al.
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Partner: UNT College of Science
open access

Visualization of Lipid Droplet Composition by Direct Organelle Mass Spectrometry

Description: Article reporting an approach that combines the visualization of individual lipid droplets (LDs), microphase extraction of lipid components from droplets, and the direct identification of lipid composition by nanospray mass spectrometry, even to the level of a single LD. The triacylglycerol (TAG) composition of LDs from several plant sources (mature cotton (Gossypium hirsutum) embryos, roots of cotton seedlings, and Arabidopsis thaliana seeds and leaves) were examined by direct organelle mass s… more
Date: February 4, 2011
Creator: Horn, Patrick J.; Ledbetter, Nicole R.; James, Christopher N.; Hoffman, William D.; Case, Charlene R.; Verbeck, Guido F. et al.
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Partner: UNT College of Science
open access

Unraveling Δ1-Pyrroline-5-Carboxylate-Proline Cycle in Plants by Uncoupled Expression of Proline Oxidation Enzymes

Description: Article showing that overexpression of MsProDH in tobacco and Arabidopsis or impairment of P5C oxidation in the Arabidopsis p5cdh mutant did not change the cellular Pro to P5C ratio under ambient and osmotic stress conditions, indicating that P5C excess was reduced to Pro in a mitochondrial-cytosolic cycle. Results show that normal oxidation of P5C to Glu by P5CDH is key to prevent P5C-Pro intensive cycling and avoid ROS production from electron run-off.
Date: September 25, 2009
Creator: Miller, Gad; Honig, Arik; Stein, Hanan; Suzuki, Nobuhiro; Mittler, Ron & Zilberstein, Aviah
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Partner: UNT College of Science
open access

Mutations in Arabidopsis Fatty Acid Amide Hydrolase Reveal That Catalytic Activity Influences Growth but Not Sensitivity to Abscisic Acid or Pathogens

Description: Article showing that the enhanced growth and NAE tolerance of the AtFAAH overexpressing seedlings depends on the catalytic activity of AtFAAH, hypersensitivity to ABA and hypersusceptibility to nonhost pathogens are independent of its enzymatic activity. Findings suggest that the AtFAAH influences plant growth and interacts with ABA signaling and plant defense through distinctly different mechanisms.
Date: December 4, 2009
Creator: Kim, Sang-Chul; Kang, Li; Nagaraj, Satish; Blancaflor, Elison B.; Mysore, Kirankumar S. & Chapman, Kent D.
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Partner: UNT College of Science
open access

A cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causes growth anomalies by disrupting brassinosteroid signaling

Description: Article on a cytochrome P450 monooxygenase commonly used for negative selection in transgenic plants causing growth anomalies by disrupting brassinosteroid signaling.
Date: April 15, 2011
Creator: Dasgupta, Kasturi; Ganesan, Savita; Manivasagam, Sindhu & Ayre, Brian G.
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Partner: UNT College of Arts and Sciences
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