Optimization of Cell Culture Procedures for Growing Neural Networks on Microelectrode Arrays
Description:
This thesis describes the development of an optimized method for culturing dissociated, monolayer neuronal networks from murine frontal cortex and midbrain. It is presented as a guidebook for use by cell culture specialists and laboratory personnel who require updated and complete procedures for use with microelectrode array (MEA) recording technology. Specific cell culture protocols, contamination prevention and control, as well common problems encountered within the cell culture facility, are…
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Date:
December 2007
Creator:
Santa Maria, Cara L.
Partner:
UNT Libraries