Function of the ENOD8 gene in nodules of Medicago truncatula.

Function of the ENOD8 gene in nodules of Medicago truncatula.

Date: December 2006
Creator: Coque, Laurent
Description: To elaborate on the function(s) of the ENOD8 gene in the nodules of M. truncatula, several different experimental approaches were used. A census of the ENOD8 genes was first completed indicating that only ENOD8.1 (nt10554-12564 of GenBank AF463407) is highly expressed in nodule tissues. A maltose binding protein-ENOD8 fusion protein was made with an E. coli recombinant system. A variety of biochemical assays were undertaken with the MBP-ENOD8 recombinant protein expressed in E. coli, which did not yield the esterase activity observed for ENOD8 protein nodule fractions purified from M. sativa, tested on general esterase substrates, α-naphthyl acetate, and p-nitrophenylacetate. Attempts were also made to express ENOD8 in a Pichia pastoris system; no ENOD8 protein could be detected from Pichia pastoris strains which were transformed with the ENOD8 expression cassette. Additionally, it was shown that the ENOD8 protein can be recombinantly synthesized by Nicotiana benthamiana in a soluble form, which could be tested for activity toward esterase substrates, bearing resemblance to nodule compounds, such as the Nod factor. Transcription localization studies using an ENOD8 promoter gusA fusion indicated that ENOD8 is expressed in the bacteroid-invaded zone of the nodule. The ENOD8 protein was also detected in that same zone by ...
Contributing Partner: UNT Libraries
Approximate Quantitative Microscopy of Pulverized Ores, Including the Use of the Camera Lucida

Approximate Quantitative Microscopy of Pulverized Ores, Including the Use of the Camera Lucida

Date: October 1919
Creator: Coghill, Will H. & Bonardi, J. P.
Description: Technical paper issued by the Bureau of Mines over microscopy of pulverized ores. As stated in the introduction, "this paper, which deals with the study of pulverized ores and the use of the camera lucida in such work, discusses an investigation undertaken at the Seattle and the Golden mining experiment stations of the Bureau of Mines" (p. 3). This paper includes tables, illustrations, and photographs.
Contributing Partner: UNT Libraries Government Documents Department
Strain fields around high-energy ion tracks in α-quartz

Strain fields around high-energy ion tracks in α-quartz

Date: 2006
Creator: Follstaedt, David M.; Norman, A. K.; Doyle, B. L. & McDaniel, Floyd Del. (Floyd Delbert), 1942-
Description: This article discusses strain fields around high-energy ion tracks in α-quartz.
Contributing Partner: UNT College of Arts and Sciences